Diethylhexyl phthalate exposure amplifies oxidant and inflammatory response in fetal hyperglycemia model predisposing insulin resistance in zebrafish embryos

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dc.authorid0000-0003-0804-1475
dc.authorid0000-0002-6274-801X
dc.contributor.authorKaplan, Gül
dc.contributor.authorBeler, Merih
dc.contributor.authorÜnal, İsmail
dc.contributor.authorKaragöz, Atakan
dc.contributor.authorEğilmezer, Gizem
dc.contributor.authorÜstündağ, Ünsal Veli
dc.contributor.authorCansız, Derya
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2024-05-13T08:42:42Z
dc.date.available2024-05-13T08:42:42Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractExposure of zebrafish embryos to glucose is a suitable model for the fetal hyperglycemia seen in gestational diabetes. Diethylhexyl phthalate (DEHP), which is considered an endocrine-disrupting chemical, is one of the most common phthalate derivatives used in stretching plastic and is encountered in every area where plastic is used in daily life. In the present study, the effects of DEHP on pathways related to insulin resistance and obesity were examined in zebrafish embryos exposed to glucose as a fetal hyperglycemia model. Zebrafish embryos were exposed to DEHP, glucose, and glucose + DEHP for 72 h post-fertilization (hpf), and developmental parameters and locomotor activities were monitored. At 72 hpf ins, lepa, ppar?, atf4a, and il-6 expressions were determined by RT-PCR. Glucose, lipid peroxidation (LPO), nitric oxide (NO) levels, glutathione S-transferase (GST), superoxide dismutase (SOD), and acetylcholine esterase (AChE) activities were measured spectrophotometrically. Compared with the control group, glucose, LPO, GST activity, il6, and atf4a expressions increased in all exposure groups, while body length, locomotor, and SOD activities decreased. While AChE activity decreased in the DEHP and glucose groups, it increased in the glucose + DEHP group. Although glucose exposure increased ppar? and lepa expressions, DEHP significantly decreased the expressions of ppar? and lepa both in the DEHP and glucose + DEHP groups. Our findings showed that DEHP amplified oxidant and inflammatory responses in this fetal hyperglycemia model, predisposing insulin resistance in zebrafish embryos.
dc.description.sponsorshipMarmara University Scientific Research Projects Commission. TYL-2020-10079.en_US
dc.identifier.citationKaplan, G., Beler, M., Ünal, İ., Karagöz, A., Eğilmezer, G., Üstündağ, Ü. V. ... Emekli Alturfan, E. (2024). Diethylhexyl phthalate exposure amplifies oxidant and inflammatory response in fetal hyperglycemia model predisposing insulin resistance in zebrafish embryos. Toxicology and Industrial Health, 40(5), 232-243. http://dx.doi.org/10.1177/07482337241238475
dc.identifier.doi10.1177/07482337241238475
dc.identifier.endpage243
dc.identifier.issn0748-2337
dc.identifier.issn1477-0393
dc.identifier.issue5
dc.identifier.pmid38467557
dc.identifier.scopus2-s2.0-85187427586
dc.identifier.scopusqualityQ3
dc.identifier.startpage232
dc.identifier.urihttp://dx.doi.org/10.1177/07482337241238475
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12445
dc.identifier.volume40
dc.identifier.wos001183200000001en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÜstündağ, Ünsal Veli
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.relation.ispartofToxicology and Industrial Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/10079
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectDiethylhexyl Phthalate
dc.subjectFetal Hyperglycemia
dc.subjectInsulin Resistance
dc.subjectZebrafish Embryos
dc.titleDiethylhexyl phthalate exposure amplifies oxidant and inflammatory response in fetal hyperglycemia model predisposing insulin resistance in zebrafish embryos
dc.typeArticle

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