Are radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

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dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-8084-7855
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaplan, Necati
dc.contributor.authorÇalışkan, Tezcan
dc.contributor.authorÖzdemir, Çiğdem
dc.contributor.authorAkyuva, Yener
dc.contributor.authorAteş, Özkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:49:44Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:49:44Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.descriptionWOS: 000456596400006
dc.descriptionPubMed ID: 30120906
dc.description.abstractIn the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. In this context, it was aimed to evaluate the effects of iopromide and gadoxetic acid, frequently used in the discography, on intact lumbar disc tissue in pharmaco-molecular and histopathological level. Primary cell cultures were prepared from the healthy disc tissue of the patients operated in the neurosurgery clinic. Except for the control group, the cultures were incubated with the indicated radio-contrast agents. Cell viability, toxicity and proliferation indices were tested at specific time intervals. The cell viability was quantitatively analysed. It was also visually rechecked under a fluorescence microscope with acridine orange/propidium iodide staining. Simultaneously, cell surface morphology was analysed with an inverted light microscope, while haematoxylin and eosin (H&E) staining methodology was used in the histopathological evaluations. The obtained data were evaluated statistically. Unlike the literature, iopromide or gadoxetic acid did not have any adverse effects on the cell viability, proliferation and toxicity (P <0.05). Although this study reveals that radio-contrast pharmaceuticals used in the discography, often used in neurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.
dc.identifier.citationKaraarslan, N., Yılmaz, İ., Özbek, H., Yaşar Şirin, D., Kaplan, N., Çalışkan, T. … Ateş, Ö. (2019). Are radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells? Basic & Clinical Pharmacology & Toxicology, 124(2), 181-189. https://dx.doi.org/10.1111/bcpt.13112
dc.identifier.doi10.1111/bcpt.13112
dc.identifier.endpage189
dc.identifier.issn1742-7835
dc.identifier.issn1742-7843
dc.identifier.issue2
dc.identifier.scopusqualityQ2
dc.identifier.startpage181
dc.identifier.urihttps://dx.doi.org/10.1111/bcpt.13112
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1739
dc.identifier.volume124
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytotoxicity
dc.subjectDiscography
dc.subjectIntact Intervertebral Disc
dc.subjectPrimary Cell Culture
dc.subjectRadio-Contrast Agents
dc.titleAre radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?
dc.typeArticle

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