Total internal reflection holographic microscopy for cellular imaging

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dc.authorid0000-0003-0176-8807
dc.contributor.authorGürcan, Tolga
dc.contributor.authorToy, Muhammed Fatih
dc.date.accessioned2022-07-19T06:33:39Z
dc.date.available2022-07-19T06:33:39Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractThe study of interfacial structures is of utmost importance not only for various research fields such as cell biology and display systems but also their sub-disciplines. One of the traditional means of imaging buried structures rely on the use optical sectioning with superresolution microscopy. Although it exceeds diffraction limit in resolution, there are various shortcomings to utilize this methodology such as its reliance on fluorescent markers, long exposure times to high cost of the imaging system. Ultimately, these limitations position the existing technologies unideal for live cell imaging, including the imaging of surface proteins of a living cell. A label free quantitative phase imaging method is realized in this project to enable imaging of an interface between different media. This system is based on an off-axis holographic microscope and uses a high numerical aperture (NA) microscope objective to achieve total internal reflection (TIR). Existing literature on total internal reflection holographic microscopy utilizes prism to achieve TIR which limits the working distance of objective hence magnification. Our system relies on a 100x objective with 1.49 NA to improve resolution and magnification. Complex field which is reflected from the sample can be recovered by using digital holography principles. The resolution of the system can further be enhanced by combining several illumination angles and utilizing synthetic aperture reconstruction.
dc.description.sponsorshipCity of Strasbourg ; CNRS ; Cube ; IdEx University of Strasbourg ; The Society of Photo-Optical Instrumentation Engineers (SPIE) ; Universite de Strasbourgen_US
dc.description.sponsorshipTurkish Academy of Sciencesen_US
dc.identifier.citationGürcan, T. ve Toy, M. F. (2022). Total internal reflection holographic microscopy for cellular imaging. Unconventional Optical Imaging III. Virtual, Online, 9-20 May 2022. http://doi.org/10.1117/12.2621555
dc.identifier.doi10.1117/12.2621555
dc.identifier.isbn9781510651487
dc.identifier.isbn9781510651494
dc.identifier.issn0277-786X
dc.identifier.issn1996-756X
dc.identifier.scopus2-s2.0-85132833152
dc.identifier.scopusqualityN/A
dc.identifier.urihttp://doi.org/10.1117/12.2621555
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9585
dc.identifier.volume12136
dc.identifier.wos000837998500003en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorToy, Muhammed Fatih
dc.language.isoen
dc.publisherSPIE
dc.relation.ispartofUnconventional Optical Imaging IIIen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/120F099
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDigital Holographic Microscopy
dc.subjectInterfacial Imaging
dc.subjectOptical Microscopy
dc.subjectTotal Internal Reflection
dc.subjectTotal Internal Reflection Microscopy
dc.titleTotal internal reflection holographic microscopy for cellular imaging
dc.typeConference Object

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