The protective effect of adipose-derived stromal vascular fraction on ovarian function in rats with cyclophosphamide-induced ovarian damage

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dc.contributor.authorÖkten, Sabri Berkem
dc.contributor.authorÖzcan, Pınar
dc.contributor.authorTok, Olgu Enis
dc.contributor.authorDevranoğlu, Belgin
dc.contributor.authorÇetin, Çağlar
dc.contributor.authorTanoğlu, Fatma Başak
dc.contributor.authorFıçıcıoğlu, Cem
dc.date.accessioned2025-10-11T13:37:00Z
dc.date.available2025-10-11T13:37:00Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractObjective: The aim of this study was to investigate if adipose-derived stromal vascular fraction (SVF) treatment has any protective effect on ovarian function in rats with cyclophosphamide (CP) induced ovarian damage. Design: This was an experimental animal study. Participants/Materials, Setting, Methods: 25 mature cycling Wistar-Albino rats were randomized into four groups (n = 5 per group). Rats in groups 1 and 2 received single dose of intraperitoneal (i.p.) 1 mL/kg sodium chloride 0.9% (NaCl). Groups 3 and 4 received single dose of 75 mg/kg i.p. CP. On seventh day, SVF was prepared from adipose tissues of 5 additional rats and groups 1 and 3 received 0.9% NaCl i.p. injections while groups 2 and 4 received 0.2 mL i.p. injections of SVF. On day 21 all rats were euthanized, and serum anti-mullerian hormone (AMH) levels, primordial, primary, secondary, antral, and atretic follicle counts, AMH positive staining follicle counts along with AMH staining intensity of the follicles were evaluated. Results: Among two CP induced ovarian damaged groups, SVF treated group showed significantly higher secondary and antral follicle and lower atretic follicle counts, significantly higher mean serum AMH levels, AMH positive antral follicle count and higher intensity of AMH positive follicle scores for primary, secondary, and antral follicles when compared to untreated group. Moreover, group 1 showed no significant difference for all parameters except antral follicle count and AMH positive staining intensity scores for antral follicles when compared to group 4. Limitations: This study was conducted on experimental rat model. Conclusion: Our study demonstrated a significant protective effect of SVF against CP-induced ovarian damage which reveals the apparent need for further investigation of its precise mechanisms of action as it may provide a new treatment approach for women with premature ovarian failure.
dc.identifier.citationÖkten, S. B., Özcan, P., Tok, O. E., Devranoğlu, B., Çetin, Ç., Tanoğlu, F. B. ... Fıçıcıoğlu, C. (2025). The protective effect of adipose-derived stromal vascular fraction on ovarian function in rats with cyclophosphamide-induced ovarian damage. Gynecologic and Obstetric Investigation, 90(2), 120-128. http://dx.doi.org/10.1159/000541049
dc.identifier.doi10.1159/000541049
dc.identifier.endpage128
dc.identifier.issn0378-7346
dc.identifier.issn1423-002X
dc.identifier.issue2
dc.identifier.pmid39265557
dc.identifier.scopus2-s2.0-85207350568
dc.identifier.scopusqualityQ2
dc.identifier.startpage120
dc.identifier.urihttp://dx.doi.org/10.1159/000541049
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13086
dc.identifier.volume90
dc.identifier.wosWOS:001329233800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTok, Olgu Enis
dc.institutionauthorid0000-0002-4899-9146
dc.language.isoen
dc.relation.ispartofGynecologic and Obstetric Investigation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFertility Preservation
dc.subjectPremature Ovarian Failure
dc.subjectPrimary Ovarian Insufficiency
dc.subjectStem Cells
dc.subjectStromal Vascular Fraction
dc.titleThe protective effect of adipose-derived stromal vascular fraction on ovarian function in rats with cyclophosphamide-induced ovarian damage
dc.typeArticle

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