Recombinant plasmid-based quantitative Real-Time PCR analysis of salmonella enterica serotypes and its application to milk samples

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dc.authorid0000-0002-0060-2859
dc.contributor.authorGökduman, Kurtuluş
dc.contributor.authorAvşaroğlu, Dilek
dc.contributor.authorÇakiriş, Aris
dc.contributor.authorÜstek, Duran
dc.contributor.authorGürakan, Candan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:58:37Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:58:37Z
dc.date.issued2016
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.descriptionWOS: 000371368200011
dc.descriptionPubMed ID: 26820062
dc.description.abstractThe aim of the current study was to develop, a new, rapid, sensitive and quantitative Salmonella detection method using a Real-Time PCR technique based on an inexpensive, easy to produce, convenient and standardized recombinant plasmid positive control. To achieve this, two recombinant plasmids were constructed as reference molecules by cloning the two most commonly used Salmonella-specific target gene regions, invA and ttrRSBC The more rapid detection enabled by the developed method (21 h) compared to the traditional culture method (90 h) allows the quantitative evaluation of Salmonella (quantification limits of 10(1) CFU/ml and 10(0) CFU/ml for the invA target and the ttrRSBC target, respectively), as illustrated using milk samples. Three advantages illustrated by the current study demonstrate the potential of the newly developed method to be used in routine analyses in the medical, veterinary, food and water/environmental sectors: I - The method provides fast analyses including the simultaneous detection and determination of correct pathogen counts; II - The method is applicable to challenging samples, such as milk; III - The method's positive controls (recombinant plasmids) are reproducible in large quantities without the need to construct new calibration curves.
dc.description.sponsorshipMiddle East Technical University (METU) [BAP-03-14-2010-05]en_US
dc.description.sponsorshipThis study was supported by Middle East Technical University (METU) research fund: BAP-03-14-2010-05.en_US
dc.identifier.citationGökduman, K., Avşaroğlu, D., Çakiriş, A., Üstek, D. ve Gürakan, C. (2016). Recombinant plasmid-based quantitative Real-Time PCR analysis of salmonella enterica serotypes and its application to milk samples. Journal Of Microbiological Methods, 122, 50-58. https://dx.doi.org/10.1016/j.mimet.2016.01.008
dc.identifier.doi10.1016/j.mimet.2016.01.008
dc.identifier.endpage58
dc.identifier.issn0167-7012
dc.identifier.issn1872-8359
dc.identifier.scopusqualityQ2
dc.identifier.startpage50
dc.identifier.urihttps://dx.doi.org/10.1016/j.mimet.2016.01.008
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3202
dc.identifier.volume122
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal Of Microbiological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSalmonella
dc.subjectInvA
dc.subjectttrRSBC
dc.subjectRecombinant Plasmids
dc.subjectReal-Time PCR
dc.subjectMilk
dc.titleRecombinant plasmid-based quantitative Real-Time PCR analysis of salmonella enterica serotypes and its application to milk samples
dc.typeArticle

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