The value of microscopic-observation drug susceptibility assay in the diagnosis of tuberculosis and detection of multidrug resistance

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dc.contributor.authorSertel Şelale, Deniz
dc.contributor.authorUzun, Meltem
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:35:37Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:35:37Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractInexpensive, rapid, and reliable tests for detecting the presence and drug susceptibility of Mycobacterium tuberculosis complex (MTBC) are urgently needed to control the transmission of tuberculosis. In this study, we aimed to assess the accuracy and speed of the microscopic-observation drug susceptibility (MODS) assay in the identification of MTBC and detection of multidrug resistance. Sputum samples from patients suspected to have tuberculosis were simultaneously tested with MODS and conventional culture [Löwenstein-Jensen (LJ) culture, BACTEC MGIT™ 960 (MGIT) system], and drug susceptibility testing (MGIT system) methods. A total of 331 sputum samples were analyzed. Sensitivity and specificity of MODS assay for detection of MTBC strains were 96% and 98.8%, respectively. MODS assay detected multidrug resistant MTBC isolates with 92.3% sensitivity and 96.6% specificity. Median time to culture positivity was similar for MGIT (8 days) and MODS culture (8 days), but was significantly longer with LJ culture (20 days) (p <0.0001 for both comparisons). Median time to availability of the susceptibility results was significantly (p <0.0001) shorter with MODS assay (8 days) than MGIT system (20 days). In conclusion, MODS is an inexpensive and rapid test with good performance characteristics for direct diagnosis of tuberculosis and detection of multidrug resistance.
dc.description.sponsorshipIstanbul Universityen_US
dc.identifier.citationSertel Şelale, D. ve Uzun, M. (2018). The value of microscopic-observation drug susceptibility assay in the diagnosis of tuberculosis and detection of multidrug resistance. APMIS, 126(1), 38-44. https://dx.doi.org/10.1111/apm.12783
dc.identifier.doi10.1111/apm.12783
dc.identifier.endpage44
dc.identifier.issn0903-4641
dc.identifier.issn1600-0463
dc.identifier.issue1
dc.identifier.scopusqualityQ2
dc.identifier.startpage38
dc.identifier.urihttps://hdl.handle.net/20.500.12511/873
dc.identifier.urihttps://dx.doi.org/10.1111/apm.12783
dc.identifier.volume126
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBlackwell Munksgaard
dc.relation.ispartofAPMISen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectIsoniazid
dc.subjectMultidrug Resistant Tuberculosis
dc.subjectMycobacterium Tuberculosis
dc.subjectRifampicin
dc.titleThe value of microscopic-observation drug susceptibility assay in the diagnosis of tuberculosis and detection of multidrug resistance
dc.typeArticle

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