Anticancer properties of phenylboronic acid in androgen-dependent (lncap) and androgen-independent (pc3) prostate cancer cells via map kinases by 2d and 3d culture methods

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dc.contributor.authorGürsoy Gürgen, Duygu
dc.contributor.authorGüneş, Arzu
dc.contributor.authorKöse, Oğuzhan
dc.contributor.authorKaplan, Arife Ahsen
dc.contributor.authorKarabulut, Seda
dc.contributor.authorTunalı, Muzaffer Başak
dc.contributor.authorKeskin, İlknur
dc.date.accessioned2026-01-29T18:40:05Z
dc.date.available2026-01-29T18:40:05Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractObjective: This study utilized three cell lines: normal prostate epithelial RWPE-1, androgen-dependent LNCaP, and androgen-independent PC3. We investigated the inhibitory effects of phenylboronic acid (PBA)’s inhibitory effect on cellular proliferation due to its ability to disrupt microtubule formation in prostate cancer cell lines. Additionally, this study aimed to assess the cytotoxic effects of PBA on prostate cancer cells using two-dimensional (2D) and three-dimensional (3D) cell culture models. Methods: The IC50 values of PBA and colchicine were determined through viability assays in 2D and 3D models. Colony formation, proliferation, and migration assays were conducted. Immunofluorescence intensity analysis of MAPKKK proteins (ERK, JNK, p38) was performed to explore the mechanism of cellular response to PBA. Results: The IC50 values were determined for each treatment group. After 48-hour of PBA treatment, migration was inhibited more effectively than with colchicine in both cancer cell lines. After 24-hour, PBA reduced colony formation and proliferation. PBA treatment for 24-hour decreased JNK expression in PC3 and LNCaP cells in 2D models. Both PBA and colchicine increased p38 expression in PC3 spheroids. PBA’s effects on cell deformation were visualized in semi-thin sections, marking the first ultrastructural observation of PBA-induced morphological defects in cancer cells. Conclusion: PBA exerts antimitotic effects by inhibiting proliferation and migration and triggers diverse metabolic responses across different cell lines. Furthermore, PBA’s low toxicity on RWPE-1 cells suggests its potential as a promising chemotherapeutic agent for future studies.
dc.description.sponsorshipIstanbul Medipol University Scientific Research Project
dc.identifier.citationGürsoy Gürgen, D., Güneş, A., Köse, O., Kaplan, A. A., Karabulut, S., Tunalı, M. B. ... Keskin, İ. (2025). Anticancer properties of phenylboronic acid in androgen-dependent (lncap) and androgen-independent (pc3) prostate cancer cells via map kinases by 2d and 3d culture methods. Anti-Cancer Agents in Medicinal Chemistry, 25(13), 899-912. http://dx.doi.org/10.2174/0118715206352302241227031015
dc.identifier.doi10.2174/0118715206352302241227031015
dc.identifier.endpage912
dc.identifier.issn1871-5206
dc.identifier.issn1875-5992
dc.identifier.issue13
dc.identifier.pmid39838675
dc.identifier.scopus2-s2.0-105005084607
dc.identifier.scopusqualityQ2
dc.identifier.startpage899
dc.identifier.urihttp://dx.doi.org/10.2174/0118715206352302241227031015
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13398
dc.identifier.volume25
dc.identifier.wosWOS:001507438900005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGüneş, Arzu
dc.institutionauthorKöse, Oğuzhan
dc.institutionauthorKarabulut, Seda
dc.institutionauthorKeskin, İlknur
dc.institutionauthorid0000-0003-2496-7673
dc.institutionauthorid0000-0003-2128-0841
dc.institutionauthorid0000-0003-3302-5004
dc.institutionauthorid0000-0002-7059-1884
dc.language.isoen
dc.relation.ispartofAnti-Cancer Agents in Medicinal Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectElectron Microscopy
dc.subjectERK
dc.subjectJNK
dc.subjectLncap
dc.subjectP38
dc.subjectPC3
dc.subjectPhenylboronic Acid
dc.subjectProstate Cancer
dc.titleAnticancer properties of phenylboronic acid in androgen-dependent (lncap) and androgen-independent (pc3) prostate cancer cells via map kinases by 2d and 3d culture methods
dc.typeArticle

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