The effects of transcranial focused ultrasound stimulation of nucleus accumbens on neuronal gene expression and brain tissue in high alcohol-preferring rats

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dc.authorid0000-0002-4899-9146
dc.authorid0000-0002-6393-1465
dc.contributor.authorDeveci, Erdem
dc.contributor.authorAkbaş, Fahri
dc.contributor.authorErgün, Arif Şanlı
dc.contributor.authorKurtulmuş, Ayşe
dc.contributor.authorKoçak, Ali Barlas
dc.contributor.authorBoyraz, Rabia Kevser
dc.contributor.authorTok, Olgu Enis
dc.contributor.authorAydın, Mehmet Şerif
dc.contributor.authorKılıç, Özge
dc.contributor.authorBozkurt, Ayhan
dc.contributor.authorUysal, Ömer
dc.contributor.authorEşrefoğlu, Mukaddes
dc.date.accessioned2023-09-13T10:44:58Z
dc.date.available2023-09-13T10:44:58Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Ruh Sağlığı ve Hastalıkları Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.description.abstractWe investigated the effect of low-intensity focused ultrasound (LIFU) on gene expression related to alcohol dependence and histological effects on brain tissue. We also aimed at determining the miRNA-mRNA relationship and their pathways in alcohol dependence-induced expression changes after focused ultrasound therapy. We designed a case-control study for 100 days of observation to investigate differences in gene expression in the short-term stimulation group (STS) and long-term stimulation group (LTS) compared with the control sham group (SG). The study was performed in our Experimental Research Laboratory. 24 male high alcohol-preferring rats 63 to 79 days old, weighing 270 to 300 g, were included in the experiment. LTS received 50-day LIFU and STS received 10-day LIFU and 40-day sham stimulation, while the SG received 50-day sham stimulation. In miRNA expression analysis, it was found that LIFU caused gene expression differences in NAc. Significant differences were found between the groups for gene expression. Compared to the SG, the expression of 454 genes in the NAc region was changed in the STS while the expression of 382 genes was changed in the LTS. In the LTS, the expression of 32 genes was changed in total compared to STS. Our data suggest that LIFU targeted on NAc may assist in the treatment of alcohol dependence, especially in the long term possibly through altering gene expression. Our immunohistochemical studies verified that LIFU does not cause any tissue damage. These findings may lead to new studies in investigating the efficacy of LIFU for the treatment of alcohol dependence and also for other psychiatric disorders.
dc.identifier.citationDeveci, E., Akbaş, F., Ergün, A. Ş., Kurtulmuş, A., Koçak, A. B., Boyraz, R. K. ... Eşrefoğlu, M. (2023). The effects of transcranial focused ultrasound stimulation of nucleus accumbens on neuronal gene expression and brain tissue in high alcohol-preferring rats. Molecular Neurobiology, 60(2), 1099-1116. https://dx.doi.org/10.1007/s12035-022-03130-9
dc.identifier.doi10.1007/s12035-022-03130-9
dc.identifier.endpage1116
dc.identifier.issn0893-7648
dc.identifier.issn1559-1182
dc.identifier.issue2
dc.identifier.pmid36417101
dc.identifier.scopus2-s2.0-85142444054
dc.identifier.scopusqualityQ1
dc.identifier.startpage1099
dc.identifier.urihttps://dx.doi.org/10.1007/s12035-022-03130-9
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11421
dc.identifier.volume60
dc.identifier.wos000886852300002en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDeveci, Erdem
dc.institutionauthorTok, Olgu Enis
dc.institutionauthorAydın, Mehmet Şerif
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Neurobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/214S249
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlcohol Dependency
dc.subjectLow-İntensity Focused Ultrasound (LIFU)
dc.subjectmiRNA
dc.subjectNeuromodulation
dc.subjectNucleus Accumbens
dc.titleThe effects of transcranial focused ultrasound stimulation of nucleus accumbens on neuronal gene expression and brain tissue in high alcohol-preferring rats
dc.typeArticle

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