An in vitro model for conditioning lesion effect

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dc.authorid0000-0003-0352-1947
dc.contributor.authorKaval Oğuz, Elif
dc.contributor.authorÖztürk, Gürkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:51:05Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:51:05Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.descriptionWOS: 000455226200004
dc.descriptionPubMed ID: 30415355
dc.description.abstractAxons of a peripheral nerve grow faster after an axotomy if it attains a prior injury a few days earlier. This is called conditioning lesion effect (CLE) and very much valued since it may provide new insights into neuron biology and axonal regeneration. There are established in vivo experimental paradigms to study CLE, however, there is a need to have an in vitro conditioning technique where CLE occurs in a maximally controlled environment. Mouse primary sensory neurons were isolated from lumbar 4-5 dorsal root ganglia and incubated at 37 degrees C on a silicon-coated watch glass that prevents cell attachment. After this conditioning period they were transferred to laminin coated culture dishes. Similar cultures were set up with freshly isolated neurons from control animals and from the animals that received a sciatic nerve cut 3days earlier. All preparations were placed on a live cell imaging microscopy providing physiological conditions and photographed for 48h. Axonal regeneration and neuronal survival was assessed. During the conditioning incubation period neurons remained in suspended aggregates and did not grow axons. The regeneration rate of the in vitro conditioned neurons was much higher than the in vivo conditioned and control preparations during the first day of normal incubation. However, higher regeneration rates were compromised by progressive substantial neuronal death in both types of conditioned cultures but not in the control preparations. By using neutralizing antibodies, we demonstrated that activity of endogenous leukemia inhibitory factor is essential for induction of CLE in this model.
dc.description.sponsorshipYuzuncu Yil University, Directorate of Scientific Research Projectsen_US
dc.description.sponsorshipThis study was supported by Yuzuncu Yl University, Directorate of Scientific Research Projects.en_US
dc.identifier.citationKaval Oğuz, E. ve Öztürk, G. (2019). An in vitro model for conditioning lesion effect. Cellular and Molecular Neurobiology, 39(1), 61-71. https://dx.doi.org/10.1007/s10571-018-0633-2
dc.identifier.doi10.1007/s10571-018-0633-2
dc.identifier.endpage71
dc.identifier.issn0272-4340
dc.identifier.issn1573-6830
dc.identifier.issue1
dc.identifier.scopusqualityQ1
dc.identifier.startpage61
dc.identifier.urihttps://dx.doi.org/10.1007/s10571-018-0633-2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2143
dc.identifier.volume39
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer/Plenum Publisher
dc.relation.ispartofCellular and Molecular Neurobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectConditioning
dc.subjectIn Vitro
dc.subjectNeuron Culture
dc.subjectAxon Regeneration
dc.subjectDegeneration
dc.subjectLeukemia Inhibitory Factor
dc.subjectLIF
dc.titleAn in vitro model for conditioning lesion effect
dc.typeArticle

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