Detailed morphological analysis of axolotl sperm

dc.authorid0000-0002-7059-1884
dc.authorid0000-0001-6698-9032
dc.authorid0000-0001-9724-6733
dc.authorid0000-0001-9416-7757
dc.authorid0000-0003-3302-5004
dc.contributor.authorKeskin, İlknur
dc.contributor.authorGürsoy Gürgen, Duygu
dc.contributor.authorAvinca, Didem
dc.contributor.authorÖzdemir, Ekrem Musa
dc.contributor.authorKeskin, Suat Utku
dc.contributor.authorKarabulut, Seda
dc.date.accessioned2021-12-03T06:28:55Z
dc.date.available2021-12-03T06:28:55Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Tıbbi Araştırma Merkezi (MEDİTAM)
dc.description.abstractThe axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson's trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengths of the midpiece, tail, and head were 8.575 µm, 356.544 µm, and 103.661 µm, respectively. In the flagellum part, the wavy membrane structure, whose function has not been explained, surrounds the tail. The data obtained from this study will constitute an important step in designing future research on the reproductive and regeneration capacity of the axolotl.
dc.identifier.citationKeskin, İ., Gürsoy Gürgen, D., Avinca, D., Özdemir, E. M., Keskin, S. U. ve Karabulut, S. (2021). Detailed morphological analysis of axolotl sperm. Korean Journal of Veterinary Research, 61(3). https://dx.doi.org/10.14405/KJVR.2021.61.E25
dc.identifier.doi10.14405/KJVR.2021.61.E25
dc.identifier.issn2466-1384
dc.identifier.issue3
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://dx.doi.org/10.14405/KJVR.2021.61.E25
dc.identifier.urihttps://hdl.handle.net/20.500.12511/8604
dc.identifier.volume61
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherKorean Society of Veterinary Science
dc.relation.ispartofKorean Journal of Veterinary Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAmbystoma Mexicanum
dc.subjectHistology
dc.subjectSpermatozoa
dc.titleDetailed morphological analysis of axolotl sperm
dc.typeArticle

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