The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development

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dc.authorid0000-0001-6781-9983
dc.contributor.authorSozen, Berna
dc.contributor.authorÖztürk, Saffet
dc.contributor.authorYaba, Aylin
dc.contributor.authorDemir, Necdet
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:41Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:41Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.descriptionWOS: 000365991800016
dc.descriptionPubMed ID: 26025760
dc.description.abstractPreimplantation embryo development is an important and unique period and is strictly controlled. This period includes a series of critical events that are regulated by multiple signal-transduction pathways, all of which are crucial in the establishment of a viable pregnancy. The p38 mitogen-activated protein kinase ( MAPK) signalling pathway is one of these pathways, and inhibition of its activity during preimplantation development has a deleterious effect. The molecular mechanisms underlying the deleterious effects of p38 MAPK suppression in early embryo development remain unknown. To investigate of the effect of p38 MAPK inhibition on late preimplantation stages in detail, we cultured 2-cell stage embryos in the presence of SB203580 for 48 h and analysed the 8-cell, morula, and blastocyst stages. We determined that prolonged inhibition of the p38 MAPK altered the expression levels of Glut1 and Glut4, decreased glucose uptake during the 8-cell to blastocyst transition, changed the expression levels of transcripts which will be important to lineage commitment, including Oct4/Pou5f1, Nanog, Sox2, and Gata6, and increased cell death in 8-16 cell stage embryos onwards. Strikingly, while the expression levels of Nanog, Gata6 and Oct4/Pou5f1 mRNAs were significantly decreased, Sox2 mRNA was increased in SB203580-treated blastocysts. Taken together, our results provide important insight into the biological processes controlled by the p38 MAPK pathway and its critical role during preimplantation development.
dc.description.sponsorshipAkdeniz Universityen_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu (TUBITAK)en_US
dc.identifier.citationSozen, B., Öztürk, S., Yaba, A. ve Demir, N. (2015). The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development. Mechanisms of Development, 138, 375-398. https://dx.doi.org/10.1016/j.mod.2015.05.002
dc.identifier.doi10.1016/j.mod.2015.05.002
dc.identifier.endpage398
dc.identifier.issn0925-4773
dc.identifier.issn1872-6356
dc.identifier.scopusqualityQ1
dc.identifier.startpage375
dc.identifier.urihttps://dx.doi.org/10.1016/j.mod.2015.05.002
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3404
dc.identifier.volume138
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.eceu-repo/grantAgreement/TUBITAK/SOBAG/113S160
dc.relation.ispartofMechanisms of Developmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPreimplantation
dc.subjectMouse
dc.subjectP38 MAPK
dc.subjectGLUT
dc.subjectLineage Segregation
dc.titleThe p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development
dc.typeArticle

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