Generating a detailed protein profile of Fasciola hepatica during the chronic stage of infection in cattle

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dc.authorid0000-0002-8814-7351
dc.contributor.authorHaçarız, Orçun
dc.contributor.authorBaykal, Ahmet Tarık
dc.contributor.authorAkgün, Mete
dc.contributor.authorKavak, Pınar
dc.contributor.authorSağıroğlu, Mahmut Şamil
dc.contributor.authorSayers, Gearoid Patrick
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:41Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:41Z
dc.date.issued2014
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.descriptionWOS: 000337616100009
dc.descriptionPubMed ID: 24733753
dc.description.abstractFasciola hepatica is a trematode helminth causing a damaging disease, fasciolosis, in ruminants and humans. Comprehensive proteomic studies broaden our knowledge of the parasite's protein profile, and provide new insights into the development of more effective strategies to deal with fasciolosis. The objective of this study was to generate a comprehensive profile of F. hepatica proteins expressed during the chronic stage of infection in cattle by building on previous efforts in this area. The approach included an improved sample preparation procedure for surface and internal layers of the parasite, the application of nano-UPLC-ESI-qTOF-MS (nano-ultra-performance LC and ESI quadrupole TOF MS) integrated with different acquisition methods and in silico database search against various protein databases and a transcript database including a new assembly of publically available EST. Of a total of 776 identified proteins, 206 and 332 were specific to the surface and internal layers of the parasite, respectively. Furthermore, 238 proteins were common to both layers, with comparative differences of 172 proteins detected. Specific proteins not previously identified in F. hepatica, but shown to be immunomodulatory or potential drug targets for other parasites, are discussed.
dc.identifier.citationHaçarız, O., Baykal, A. T., Akgün, M., Kavak, P., Sağıroğlu, M. Ş. ve Sayers, G. P. (2014). Generating a detailed protein profile of Fasciola hepatica during the chronic stage of infection in cattle. Proteomics, 14(12), 1519-1530. https://dx.doi.org/10.1002/pmic.201400012
dc.identifier.doi10.1002/pmic.201400012
dc.identifier.endpage1530
dc.identifier.issn1615-9853
dc.identifier.issn1615-9861
dc.identifier.issue12
dc.identifier.scopusqualityQ1
dc.identifier.startpage1519
dc.identifier.urihttps://dx.doi.org/10.1002/pmic.201400012
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3710
dc.identifier.volume14
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-Blackwell
dc.relation.ispartofProteomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectFasciola Hepatica
dc.subjectFraction
dc.subjectMicrobiology
dc.subjectTegument
dc.subjectTrematode
dc.titleGenerating a detailed protein profile of Fasciola hepatica during the chronic stage of infection in cattle
dc.typeArticle

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