Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro

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dc.contributor.authorTuncer, Safa
dc.contributor.authorDemirci, Mustafa
dc.contributor.authorSchweikl, Helmut
dc.contributor.authorErgüven, Mine
dc.contributor.authorBilir, Ayhan
dc.contributor.authorKara-Tuncer, Aysun
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:51:24Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:51:24Z
dc.date.issued2012
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Ana Bilim Dalı
dc.descriptionWOS: 000311362300016
dc.descriptionPubMed ID: 22222515
dc.description.abstractObjectives The influence of dentin adhesive systems (Scotchbond Multi-Purpose, XP Bond, Xeno V, Clearfil Protect Bond, AdheSE) on cell survival, viability and proliferation was characterized after direct and indirect exposure using different cell culture techniques. Materials and methods The primers and cured bonding parts were directly exposed to cells using cell culture inserts, and complete materials were analyzed in a dentin barrier test. Cell responses were examined in 3T3 mouse fibroblasts after 24- and 72-h exposure periods by the estimation of total cell numbers (survival), apoptosis (viability) and cell proliferation. Results Cell numbers were effectively reduced by the primers of AdheSE, Protect Bond, and Scotchbond Multi-Purpose as well as XP bond after direct exposure in a cell culture insert test device. Likewise, Scotchbond Multi-Purpose primer induced a rate of apoptosis (93.9%) even higher than detected with Protect Bond primer (91.6%). Cell proliferation was entirely inhibited by primers and by Xp Bond as well. The Scotchbond Multi-Purpose was most cytotoxic in a dentin barrier test device after a 24-h indirect exposure. It also increased the percentage of cells in apoptosis to 15.4% compared to untreated controls. Conclusion Unpolymerized primers of dentin adhesives were more cytotoxic than polymerized bonding counterparts. Moreover, total etch dentin adhesives were more cytotoxic than self-etch adhesives. Clinical relevance When dentin adhesives are used in deep cavities without a protective dentin barrier the leachable hydrophobic and hydrophilic component of dentin adhesive systems can penetrate to the pulp and may induce cytotoxic responses in pulp tissues.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University [3230]en_US
dc.description.sponsorshipThe current study is based on a thesis submitted to the graduate faculty, University of Istanbul, in partial fulfillment of the requirements for the degree of Doctor of Philosophy and was supported by Scientific Research Projects Coordination Unit of Istanbul University. Project number 3230.en_US
dc.identifier.citationTuncer, S., Demirci, M., Schweikl, H., Erguven, M., Bilir, A. ve Kara-Tuncer, A.(2012). Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro. Clinical Oral Investigations, 16(6), 1635-1646. https://dx.doi.org/10.1007/s00784-011-0669-x
dc.identifier.doi10.1007/s00784-011-0669-x
dc.identifier.endpage1646
dc.identifier.issn1432-6981
dc.identifier.issue6
dc.identifier.scopusqualityQ1
dc.identifier.startpage1635
dc.identifier.urihttps://dx.doi.org/10.1007/s00784-011-0669-x
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2211
dc.identifier.volume16
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofClinical Oral Investigationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDentin Adhesive System
dc.subjectBiocompatibility
dc.subjectCytotoxicity
dc.subjectCell Culture
dc.subjectApoptosis
dc.titleInhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro
dc.typeArticle

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