Neuroprotective effects of mitoquinone and oleandrin on Parkinson’s disease model in zebrafish

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dc.authorid0000-0003-0804-1475
dc.contributor.authorÜnal, İsmail
dc.contributor.authorÇalışkan Ak, Esin
dc.contributor.authorÜstündağ, Ünsal Veli
dc.contributor.authorAteş, Perihan Seda
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorAltınöz, Meriç Adil
dc.contributor.authorElmacı, İlhan
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2019-12-26T05:56:41Z
dc.date.available2019-12-26T05:56:41Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractAim: The aim of this study is to investigate the possible protective effects of mitoquinone and oleandrin on rotenone induced Parkinson’s disease in zebrafish. Materials and methods: Adult zebrafish were exposed to rotenone and mitoquinone for 30 days. Biochemical parameters were determined by spectrophotometric method and Parkinson’s disease-related gene expressions were determined by reverse transcription polymerase chain reaction method. Measurement of neurotransmitters was performed by liquid chromatography tandem-mass spectrometry instrument. The accumulation of synuclein was demonstrated by immunohistochemical staining. In vitro thiazolyl blue tetrazolium bromide method was applied to determine the mitochondrial function of synaptosomal brain fractions using rotenone as a neurotoxic agent and mitoquinone and oleandrin as neuroprotective agents. Results: Mitoquinone improved the oxidant-antioxidant balance and neurotransmitter levels that were disrupted by rotenone. Mitoquinone also ameliorated the expressions of Parkinson’s disease-related gene expressions that were disrupted by rotenone. According to thiazolyl blue tetrazolium bromide assay results, mitoquinone and oleandrin increased mitochondrial function which was decreased due to rotenone exposure. Conclusion: Based on the results of our study, positive effects of mitoquinone were observed in Parkinson’s disease model induced by rotenone in zebrafish.
dc.identifier.citationÜnal, İ., Çalışkan-Ak, E., Üstündağ, Ü. V., Ateş, P. S., Alturfan, A. A., Altınöz, M. A. ... Emekli-Alturfan, E.(2020). Neuroprotective effects of mitoquinone and oleandrin on Parkinson’s disease model in zebrafish. International Journal of Neuroscience, 130(6), 574-582. https://doi.org/10.1080/00207454.2019.1698567
dc.identifier.doi10.1080/00207454.2019.1698567
dc.identifier.endpage582
dc.identifier.issn0020-7454
dc.identifier.issn1543-5245
dc.identifier.issue6
dc.identifier.scopusqualityQ2
dc.identifier.startpage574
dc.identifier.urihttps://doi.org/10.1080/00207454.2019.1698567
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4704
dc.identifier.volume130
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofInternational Journal of Neuroscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectZebrafish
dc.subjectParkinson’s Disease
dc.subjectMitoquinone
dc.subjectOleandrin
dc.titleNeuroprotective effects of mitoquinone and oleandrin on Parkinson’s disease model in zebrafish
dc.typeArticle

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