Culturing, freezing, processing, and imaging of entire organoids and spheroids while still in a hydrogel

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dc.authorid0000-0002-4899-9146
dc.contributor.authorTok, Olgu Enis
dc.contributor.authorDemirel, Gamze
dc.contributor.authorSaatçi, Yusuf
dc.contributor.authorAkbulut, Zeynep
dc.contributor.authorKayalar, Özgecan
dc.contributor.authorAktaş, Ranan Gülhan
dc.date.accessioned2023-01-13T10:15:11Z
dc.date.available2023-01-13T10:15:11Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractOrganoids and spheroids, three-dimensional growing structures in cell culture labs, are becoming increasingly recognized as superior models compared to two-dimensional culture models, since they mimic the human body better and have advantages over animal studies. However, these studies commonly face problems with reproducibility and consistency. During the long experimental processes-with transfers of organoids and spheroids between different cell culture vessels, pipetting, and centrifuging-these susceptible and fragile 3D growing structures are often damaged or lost. Ultimately, the results are significantly affected, since the 3D structures cannot maintain the same characteristics and quality. The methods described here minimize these stressful steps and ensure a safe and consistent environment for organoids and spheroids throughout the processing sequence while they are still in a hydrogel in a multipurpose device. The researchers can grow, freeze, thaw, process, stain, label, and then examine the structure of organoids or spheroids under various high-tech instruments, from confocal to electron microscopes, using a single multipurpose device. This technology improves the studies' reproducibility, reliability, and validity, while maintaining a stable and protective environment for the 3D growing structures during processing. In addition, eliminating stressful steps minimizes handling errors, reduces time taken, and decreases the risk of contamination.
dc.description.sponsorshipIstanbul Medipol University Research Institute for Health Sciences and Technologies ; Maltepe University ; University of Chicagoen_US
dc.identifier.citationTok, O. E., Demirel, G., Saatçi, Y., Akbulut, Z., Kayalar, Ö. ve Aktaş, R. G. (2022). Culturing, freezing, processing, and imaging of entire organoids and spheroids while still in a hydrogel. Journal of Visualized Experiments, 2022(190). https://dx.doi.org/10.3791/64563
dc.identifier.doi10.3791/64563
dc.identifier.issn1940-087X
dc.identifier.issue190
dc.identifier.pmid36622008
dc.identifier.scopus2-s2.0-85145275357
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.3791/64563
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10307
dc.identifier.volume2022
dc.identifier.wos000920277000008en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTok, Olgu Enis
dc.institutionauthorSaatçi, Yusuf
dc.language.isoen
dc.publisherJournal of Visualized Experiments
dc.relation.ispartofJournal of Visualized Experimentsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHydrogel
dc.subjectEntire Organoids
dc.subjectProcessing
dc.subjectCulturing
dc.titleCulturing, freezing, processing, and imaging of entire organoids and spheroids while still in a hydrogel
dc.typeArticle

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