Dual action of exosomes derived from in vitro A beta toxicity model: The role of age for pathological response

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dc.authorid0000-0001-9400-187X
dc.authorid0000-0002-0323-6153
dc.authorid0000-0002-4051-2559
dc.authorid0000-0002-9476-8488
dc.contributor.authorBeker, Merve
dc.contributor.authorGünay, Necmeddin
dc.contributor.authorSarıkamış, Bahar
dc.contributor.authorKalkan Çakmak, Rabia
dc.contributor.authorErçin, Nilüfer
dc.contributor.authorAltıntaş, Mehmet Özgen
dc.contributor.authorAltunay, Serdar
dc.contributor.authorBeker, Mustafa Çağlar
dc.contributor.authorSarı Ak, Duygu
dc.contributor.authorKılıç, Ülkan
dc.date.accessioned2023-01-12T06:55:01Z
dc.date.available2023-01-12T06:55:01Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Sinirbilim Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.description.abstractExosomes released from different cell types of the central nervous system play an essential role in the patho-genesis of Alzheimer's disease (AD). In this study, we aimed to create an animal model by injecting exosomes that carry AD markers into the brain to shed light on the mechanism behind Alzheimer's pathology. Exosomes obtained from mouse Neuro2A, to which A beta toxicity model applied, were used as a mediator to build an AD phenotype. For this purpose, exosomes were administered into hippocampal CA3 region of mice with different ages. Firstly, the possible role of exosomes on brain volume was analyzed. Then, neurons and astrocytes were evaluated for survival. In addition, the progenitor cells' differentiation capacity was investigated via BrdU staining. AKT signaling pathway components were examined to detect the molecular mechanisms behind the exosomal function. We found different responses in different age groups. Expression of APP upregulated only in young animals upon delivery of A beta-exosomes. Interestingly, young animals represented increased numbers of neurons in the hippocampus, and neurogenesis was found to be restricted after A beta-Ex injections. However, in relation to exosome administration, the glial intensity increased in aged animals. Lastly, phosphorylation of survival kinase AKT was downregulated due to the presence of A beta in both young and old animals. The findings reveal that the exosomes from an in vitro A beta toxicity model may induce different responses in an age-dependent manner. This study is the first to report the relationship between exosomal function and aging by evaluating the key molecules.
dc.identifier.citationBeker, M., Günay, N., Sarıkamış, B., Kalkan Çakmak, R., Erçin, N., Altıntaş, M. Ö. ... Kılıç, Ü. (2023). Dual action of exosomes derived from in vitro A beta toxicity model: The role of age for pathological response. Archives of Gerontology and Geriatrics, 106. https://dx.doi.org/10.1016/j.archger.2022.104874
dc.identifier.doi10.1016/j.archger.2022.104874
dc.identifier.issn0167-4943
dc.identifier.issn1872-6976
dc.identifier.pmid36470179
dc.identifier.scopus2-s2.0-85143177137
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1016/j.archger.2022.104874
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10289
dc.identifier.volume106
dc.identifier.wos000897712900010en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGünay, Necmeddin
dc.institutionauthorAltıntaş, Mehmet Özgen
dc.institutionauthorAltunay, Serdar
dc.institutionauthorBeker, Mustafa Çağlar
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofArchives of Gerontology and Geriatricsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAmyloid ?
dc.subjectExosome
dc.subjectHippocampus
dc.subjectNeurogenesis
dc.subjectSurvival
dc.titleDual action of exosomes derived from in vitro A beta toxicity model: The role of age for pathological response
dc.title.alternativeDual action of exosomes derived from in vitro A? toxicity model: The role of age for pathological response
dc.typeArticle

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