Quantitative measurement of HER2/neu oncogene amplification and p53 tumor suppressor gene deletion by RT-PCR in breast cancer

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dc.authorid0000-0003-2350-6381
dc.contributor.authorÜlfer, Gözde
dc.date.accessioned2024-01-17T08:29:10Z
dc.date.available2024-01-17T08:29:10Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractObjective: The aim of the study was to quantitatively evaluate HER2/neu oncogene amplification and p53 tumor suppressor gene deletion in breast cancer with real-time polymerase chain reaction (RT-PCR). Materials and Methods: Sections obtained from the tumor tissues of 50 patients were paraffinized on slides, and DNA extraction was performed. HER2/neu amplification and p53 deletion were analyzed using RT-PCR with respect to immunohistochemistry (IHC). Results: For 25 patients with breast cancer, we compared IHC and RT-PCR results for the quantitative measurement of HER2/neu expression. We found a significant correlation between the results obtained using IHC and RT-PCR (p < 0.05). Taking the results of IHC as reference, the sensitivity and specificity of the RT-PCR method were 57% and 83%, respectively. HER2/neu amplification and p53 deletion did not have a significant correlation with tumor size, histological grade, lymph node invasion, and status of estrogen receptor and progesterone receptor (p>0.05, for all). Conclusion: RT-PCR measured gene levels reliably and accurately with high sensitivity and specificity, making it superior to IHC, which is subjective.
dc.identifier.citationÜlfer, G. (2023). Quantitative measurement of HER2/neu oncogene amplification and p53 tumor suppressor gene deletion by RT-PCR in breast cancer. Experimed, 13(3), 257-262. https://dx.doi.org/10.26650/experimed.1374959
dc.identifier.doi10.26650/experimed.1374959
dc.identifier.endpage262
dc.identifier.issn2667-5846
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85180920115
dc.identifier.scopusqualityQ4
dc.identifier.startpage257
dc.identifier.urihttps://dx.doi.org/10.26650/experimed.1374959
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12158
dc.identifier.volume13
dc.identifier.wosWOS:001322033400010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorÜlfer, Gözde
dc.language.isoen
dc.publisherIstanbul University Press
dc.relation.ispartofExperimeden_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBreast Cancer
dc.subjectHER2/neu
dc.subjectp53
dc.subjectRT-PCR
dc.titleQuantitative measurement of HER2/neu oncogene amplification and p53 tumor suppressor gene deletion by RT-PCR in breast cancer
dc.typeArticle

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