The effect of calpain inhibitor-I on copper oxide nanoparticle-induced damage and cerebral ischemia-reperfusion in a rat model

Basit Öğe Kaydı
dc.authorid0000-0002-0675-1839
dc.authorid0000-0001-7933-4262
dc.authorid0000-0002-0109-5086
dc.contributor.authorKarimkhani, Hadi
dc.contributor.authorShojaolsadati, Paria
dc.contributor.authorYiğitbaşı, Türkan
dc.contributor.authorKolbaşı, Bircan
dc.contributor.authorEmekli, Neslin
dc.date.accessioned2024-05-17T10:44:05Z
dc.date.available2024-05-17T10:44:05Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractThis study aimed to investigate the effects of the calpain inhibitor N-Acetyl-Leu-Leu-norleucinal (ALLN) on neuroapoptotic cell damage caused by Copper Oxide Nanoparticles (CuO-NP) and exacerbation of damage through brain ischemia/reperfusion (I/R) in a rat model. Male Wistar Albino rats (n=80) were divided into eight groups: Control, I/R, CuO-NP, CuO-NP+I/R, I/R+ALLN, CuO-NP+ALLN, CuO-NP+I/R+ALLN, and DMSO. Biochemical markers (MBP, S100B, NEFL, NSE, BCL-2, Cyt-C, Calpain, TNF-?, Caspase-3, MDA, and CAT) were measured in serum and brain tissue samples. Histological examinations (H&E staining), DNA fragmentation analysis (TUNEL) were performed, along with Caspase-3 assessment. The ALLN-treated groups exhibited significant improvements in biochemical markers and a remarkable reduction in apoptosis compared to the damaged groups (CuO-NP and I/R). H&E and Caspase-3 staining revealed damage-related morphological changes and reduced apoptosis in the ALLN-treated group. However, no differences were observed among the groups with TUNEL staining. The findings suggest that ALLN, as a calpain inhibitor, has potential implications for anti-apoptotic treatment, specifically in mitigating neuroapoptotic cell damage caused by CuO-NP and I/R.
dc.identifier.citationKarimkhani, H., Shojaolsadati, P., Yiğitbaşı, T., Kolbaşı, B. ve Emekli, N. (2024). The effect of calpain inhibitor-I on copper oxide nanoparticle-induced damage and cerebral ischemia-reperfusion in a rat model. Biomedicine and Pharmacotherapy, 174. http://dx.doi.org/10.1016/j.biopha.2024.116539
dc.identifier.doi10.1016/j.biopha.2024.116539
dc.identifier.issn0753-3322
dc.identifier.pmid38615610
dc.identifier.scopus2-s2.0-85190292716
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1016/j.biopha.2024.116539
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12478
dc.identifier.volume174
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYiğitbaşı, Türkan
dc.institutionauthorKolbaşı, Bircan
dc.institutionauthorEmekli, Neslin
dc.language.isoen
dc.relation.ispartofBiomedicine and Pharmacotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAnti-Apoptotic Treatment
dc.subjectBrain İschemia-Reperfusion (I/R)
dc.subjectCalpain Inhibitor
dc.subjectCerebral İschemia-Reperfusion (I/R)
dc.subjectCopper Oxide Nanoparticle (Cuo-NP)
dc.subjectRat Model
dc.titleThe effect of calpain inhibitor-I on copper oxide nanoparticle-induced damage and cerebral ischemia-reperfusion in a rat model
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Yiğitbaşı-Türkan-2024.pdf
Boyut:
6.23 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu