Refractive index tomography of myelinating glial cells

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dc.authorid0000-0003-0176-8807
dc.authorid0000-0003-1106-3288
dc.contributor.authorToy, Muhammed Fatih
dc.contributor.authorKurt Vatandaşlar, Burcu
dc.contributor.authorKerman, Bilal Ersen
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:47Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:47Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.descriptionConference on Quantitative Phase Imaging V -- FEB 02-05, 2019 -- San Francisco, CA
dc.descriptionWOS: 000471821600014
dc.description.abstractRefractive index tomography as an emerging technique enables the 3D morphological investigation of cells with no marker. Here, refractive index tomographic imaging of myelinating glial cells is presented. Myelin as a signal insulation layer around an axon is formed by the wrapping of Schwann cells or oligodendrocytes. Microscopic investigation of myelination traditionally requires fluorescent markers. Glial cells generally wrap the axon for more than ten layers. This multilayer formation has alternating and uniform layers of protein and lipid. Earlier studies on the structure of the myelin sheath have shown that the thickness period is lower than 20nm including the thickness of the extracellular medium after each layer. Direct observation of an individual layer is not possible (using classical microscopy techniques) due to dimensions being very small compared to the wavelength of the illumination light. However, periodic nature of the layers enables the differentiation of a myelinated axon from an unmyelinated one. Rapid change of the integrated refractive index and the Bragg fiber like structure alters the transmission behavior as a function of wavelength and incidence angle. With the 3D sectioning capability of refractive index tomography, these features can be easily identified.
dc.description.sponsorshipSPIE, Tomocube, Inc, Phi Optics, Incen_US
dc.description.sponsorshipScientific and Technological Council of Turkey (TUBITAK) [116F437]; Turkish Academy of Sciencesen_US
dc.description.sponsorshipThis work is supported by the Scientific and Technological Council of Turkey (TUBITAK) under grant No. 116F437. B. E. Kerman gratefully acknowledges the support of the Turkish Academy of Sciences.en_US
dc.identifier.citationToy, M. F., Kurt Vatandaşlar, B. ve Kerman, B. E. (2019). Refractive index tomography of myelinating glial cells. Conference on Quantitative Phase Imaging V. San Francisco, United States, February 02-05, 2019. https://dx.doi.org/10.1117/12.2512706
dc.identifier.doi10.1117/12.2512706
dc.identifier.isbn9781510624177
dc.identifier.issn1605-7422
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://dx.doi.org/10.1117/12.2512706
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3733
dc.identifier.volume10887
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSPIE-Int Soc Optical Engineering
dc.relation.ispartofConference on Quantitative Phase Imaging Ven_US
dc.relation.ispartofseriesProgress in Biomedical Optics and Imaging
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRefractive Index Tomography
dc.subjectQuantitative Phase Tomography
dc.subjectMyelin
dc.titleRefractive index tomography of myelinating glial cells
dc.typeConference Object

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