Development of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis

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dc.authorid0000-0001-8022-7325
dc.contributor.authorKolukırık, Mustafa
dc.contributor.authorYılmaz, Mesut
dc.contributor.authorİnce, Orhan
dc.contributor.authorKetre, Canan
dc.contributor.authorTosun İstanbullu, Ayşe
dc.contributor.authorİnce Kasapgil, Bahar
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:36:22Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:36:22Z
dc.date.issued2016
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractBackground: Group A streptococci (GAS) are the most common bacterial cause of acute pharyngitis and account for 15-30 % of cases of acute pharyngitis in children and 5-10 % of cases in adults. In this study, a real-time quantitative PCR (qPCR) based GAS detection assay in pharyngeal swab specimens was developed. Methods: The qPCR assay was compared with the gold standard bacterial culture and a rapid antigen detection test (RADT) to evaluate its clinical performance in 687 patients. The analytical sensitivity of the assay was 240 cfu/swab. Forty-five different potential cross-reacting organisms did not react with the test. Four different laboratories for the reproducibility studies were in 100 % (60/60) agreement for the contrived GAS positive and negative swab samples. Results: The relative sensitivities of the RADT and the qPCR test were 55.9 and 100 %; and the relative specificities were 100 and 96.3 %, respectively. Duration of the total assay for 24 samples including pre-analytical processing and analysis changed between 42 and 55 min depending on the type of qPCR instrument used. A simple DNA extraction method and a low qPCR volume made the developed assay an economical alternative for the GAS detection. Conclusion: We showed that the developed qPCR test is rapid, cheap, sensitive and specific and therefore can be used to replace both antigen detection and culture for diagnosis of acute GAS pharyngitis.
dc.identifier.citationKolukırık, M., Yılmaz, M., İnce, O., Ketre, C., Tosun İstanbullu, A. ve İnce Kasapgil, B. (2016). Development of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis. Annals of Clinical Microbiology and Antimicrobials, 15(1), 2-6. https://dx.doi.org/10.1186/s12941-016-0162-0
dc.identifier.doi10.1186/s12941-016-0162-0
dc.identifier.endpage6
dc.identifier.issn1476-0711
dc.identifier.issue1
dc.identifier.scopusqualityQ1
dc.identifier.startpage2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1144
dc.identifier.urihttps://dx.doi.org/10.1186/s12941-016-0162-0
dc.identifier.volume15
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.relation.ispartofAnnals of Clinical Microbiology and Antimicrobialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAcute Pharyngitis
dc.subjectGroup A Streptococci
dc.subjectQPCR
dc.subjectRapid Antigen Detection Test
dc.titleDevelopment of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis
dc.typeArticle

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