Bisphenol a and di(2-ethylhexyl) phthalate exert divergent effects on apoptosis and the Wnt/-catenin pathway in zebrafish embryos: A possible mechanism of endocrine disrupting chemical action

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dc.authorid0000-0002-0675-1839
dc.authorid0000-0003-0804-1475
dc.contributor.authorÜstündağ, Ünsal Veli
dc.contributor.authorÜnal, İsmail
dc.contributor.authorAteş, Perihan Seda
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorYiğitbaşı, Türkan
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:03:17Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:03:17Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.descriptionWOS: 000416824500003
dc.descriptionPubMed ID: 28992791
dc.description.abstractPolyethylene terephthalate (PET) and polycarbonate (PC) are the most commonly used plastics in water bottles. Di(2-ethylhexyl) phthalate (DEHP) is used as a plasticizer in PET plastics, and bisphenol A (BPA) is used to produce PC. Both DEHP and BPA are known for their potential endocrine disrupting effects. The Wnt/-catenin signaling pathway has important roles in cell proliferation, cell specification and cell fate determination during embryonic development. Recent reports suggest a link between the Wnt/-catenin signaling pathway and apoptosis. The aim of this study was to investigate the relation between Wnt/-catenin signaling and apoptosis in the case of BPA and DEHP exposure in zebrafish embryos. Accordingly, in vivo cell death was assessed using acridine orange staining, and reverse transcription polymerase chain reaction was used to determine the expressions of wnt3a, gsk3 and ccnd1. Proliferative cell nuclear antigen, -catenin and Wnt3a expressions were determined immunohistochemically. Vitellogenin levels were determined using Enzyme Linked ImmunoSorbent Assay (ELISA). Increased vitellogenin levels, apoptosis, and wnt3a and gsk3 expressions were observed in BPA-exposed zebrafish embryos. Increased apoptosis in the BPA-exposed embryos may be due to the pro-apoptotic changes induced by Wnt3a, whereas DEHP might be suggested to have a minor effect as Wnt3a expression; vitellogenin levels and apoptosis did not increase significantly following exposure to DEHP.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [114S537]; Marmara University Scientific Research Projects Commission [SAG-E-120314-0056]en_US
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This project was supported by TUBITAK (The Scientific and Technological Research Council of Turkey), Project Number: 114S537 and by Marmara University Scientific Research Projects Commission, Project No: SAG-E-120314-0056.en_US
dc.identifier.citationÜstündağ, Ü. V., Ünal, İ., Ateş, P. S., Alturfan, A. A., Yiğitbaşı, T. ve Emekli Alturfan, E. (2017). Bisphenol a and di(2-ethylhexyl) phthalate exert divergent effects on apoptosis and the Wnt/-catenin pathway in zebrafish embryos: A possible mechanism of endocrine disrupting chemical action. Toxicology and Industrial Health, 33(12), 901-910. https://dx.doi.org/10.1177/0748233717733598
dc.identifier.doi10.1177/0748233717733598
dc.identifier.endpage910
dc.identifier.issn0748-2337
dc.identifier.issn1477-0393
dc.identifier.issue12
dc.identifier.scopusqualityQ3
dc.identifier.startpage901
dc.identifier.urihttps://dx.doi.org/10.1177/0748233717733598
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3842
dc.identifier.volume33
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Inc
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/114S537
dc.relation.ispartofToxicology and Industrial Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectDi(2-Ethylhexyl) Phthalate
dc.subjectBisphenol A
dc.subjectApoptosis
dc.subjectWnt
dc.subjectCatenin
dc.subjectZebrafish Embryo
dc.titleBisphenol a and di(2-ethylhexyl) phthalate exert divergent effects on apoptosis and the Wnt/-catenin pathway in zebrafish embryos: A possible mechanism of endocrine disrupting chemical action
dc.typeArticle

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