Expression of pro-apoptotic and anti-apoptotic proteins in granulosa cells of women with diminished ovarian reserve

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dc.authorid0000-0002-2765-5766
dc.authorid0000-0002-7059-1884
dc.contributor.authorBulgurcuoğlu Kuran, Sibel
dc.contributor.authorAltun, Ayşe
dc.contributor.authorKaramustafaoğlu Balcı, Burçin
dc.contributor.authorKeskin, İlknur
dc.contributor.authorHocaoğlu, Meryem
dc.date.accessioned2022-11-22T07:25:10Z
dc.date.available2022-11-22T07:25:10Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractPurpose To evaluate the expressions of caspase-3 and cytochrome c and heat shock protein 70 (Hsp70) in granulosa cells (GCs) from women with normal ovarian reserve (NOR) and diminished ovarian reserve (DOR) undergoing intracytoplasmic sperm injection (ICSI). Methods GCs were collected from 117 infertile women during oocyte retrieval. Patients were classified into four groups as follows: DOR-COC score of 0, DOR-COC score of I, NOR-COC score of 0, and NOR-COC score of I. The caspase-3, cytochrome c, and Hsp70 analyses were performed immunohistochemically in GCs. The ICSI outcomes were evaluated prospectively. Results The clinical pregnancy and live birth rates were higher in DOR-COC score of I (15, 30.6%; 14, 38.9%) and NOR-COC score of I (19, 38.77%; 19, 52.7%) groups, compared with DOR-COC score of 0 (12, 24.4%; 3, 6.1%) and NOR-COC score of 0 (3, 6.1%; 0%) groups (p = 0.0001; 0.00002), respectively. Caspase-3 and cytochrome c expression levels were higher in DOR-COC score of 0 (23, 65.7%; 25, 71.4%) and NOR-COC score of 0 groups (19, 61.3%; 20, 64.5%), compared with DOR-COC score of I (8, 32%; 9, 36%) and NOR-COC score of I groups (7, 26.9%; 8, 30.8%) (p = 0.00297; p = 0.002), respectively. Lower expression levels of Hsp70 were found in DOR-COC score of 0 (11, 31.4%) and NOR-COC score of 0 groups (10, 32.3%), compared with DOR-COC score of I (16, 64%) and NOR-COC score of I groups (20, 76.9%) (p = 0.001), respectively. Hsp70 expression levels were positively correlated with the number of day 3 good-quality embryo and negatively correlated with estradiol levels in the DOR group. Conclusion Our data suggest that COC score of 0 is associated with increased expression levels of apoptotic proteins, decreased expression levels of anti-apoptotic protein, and poor ICSI clinical outcomes in women with and without DOR.
dc.identifier.citationBulgurcuoğlu Kuran, S., Altun, A., Karamustafaoğlu Balcı, B., Keskin, İ. ve Hocaoğlu, M. (2022). Expression of pro-apoptotic and anti-apoptotic proteins in granulosa cells of women with diminished ovarian reserve. Journal of Assisted Reproduction and Genetics, 39(3), 765-775. https://doi.org/10.1007/s10815-022-02422-2
dc.identifier.doi10.1007/s10815-022-02422-2
dc.identifier.endpage775
dc.identifier.issn1058-0468
dc.identifier.issn1573-7330
dc.identifier.issue3
dc.identifier.pmid35142968
dc.identifier.scopus2-s2.0-85124557428
dc.identifier.scopusqualityQ1
dc.identifier.startpage765
dc.identifier.urihttps://doi.org/10.1007/s10815-022-02422-2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10005
dc.identifier.volume39
dc.identifier.wos000753816800001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAltun, Ayşe
dc.institutionauthorKeskin, İlknur
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectCaspase-3
dc.subjectCumulus–Oocyte Complex
dc.subjectCytochrome C
dc.subjectDiminished Ovarian Reserve
dc.subjectHeat Shock Protein 70
dc.subjectIn Vitro Fertilization
dc.titleExpression of pro-apoptotic and anti-apoptotic proteins in granulosa cells of women with diminished ovarian reserve
dc.typeArticle

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