Metastasis suppressor proteins in cutaneous squamous cell carcinoma

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dc.authorid0000-0002-3247-613X
dc.contributor.authorBozdoğan, Önder
dc.contributor.authorVargel, İbrahim
dc.contributor.authorÇavuşoğlu, Tarık
dc.contributor.authorKarabulut, Ayşe Anıl
dc.contributor.authorKarahan, Gurbet
dc.contributor.authorSayar, Nilufer
dc.contributor.authorAtasoy, Pınar
dc.contributor.authorYuluğ, Işık
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:23Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:23Z
dc.date.issued2016
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000379274300004
dc.descriptionPubMed ID: 27215390
dc.description.abstractCutaneous squamous cell carcinomas (cSCCs) are common human carcinomas. Despite having metastasizing capacities, they usually show less aggressive progression compared to squamous cell carcinoma (SCC) of other organs. Metastasis suppressor proteins (MSPs) are a group of proteins that control and slow-down the metastatic process. In this study, we established the importance of seven well-defined MSPs including NDRG1, NM23-H1, RhoGDI2, E-cadherin, CD82/KAI1, MKK4, and AKAP12 in cSCCs. Protein expression levels of the selected MSPs were detected in 32 cSCCs, 6 in situ SCCs, and two skin cell lines (HaCaT, A-431) by immunohistochemistry. The results were evaluated semi-quantitatively using the HSCORE system. In addition, mRNA expression levels were detected by qRT-PCR in the cell lines. The HSCOREs of NM23-H1 were similar in cSCCs and normal skin tissues, while RGHOGDI2, E-cadherin and AKAP12 were significantly downregulated in cSCCs compared to normal skin. The levels of MKK4, NDRG1 and CD82 were partially conserved in cSCCs. In stage I SCCs, nuclear staining of NM23-H1 (NM23-H1nuc) was significantly lower than in stage SCCs. Only nuclear staining of MKK4 (MKK4nuc) showed significantly higher scores in in situ carcinomas compared to invasive SCCs. In conclusion, similar to other human tumors, we have demonstrated complex differential expression patterns for the MSPs in in-situ and invasive cSCCs. This complex MSP signature warrants further biological and experimental pathway research.
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK) [SBAG-108S184]en_US
dc.description.sponsorshipThis study was financially supported by the Scientific and Technical Research Council of Turkey (TUBITAK, grant number SBAG-108S184). The project was approved by the Kirikkale University Local Ethics Committee (07.04.2008/2008-039).en_US
dc.identifier.citationBozdoğan, Ö., Vargel, İ., Çavuşoğlu, T., Karabulut, A. A., Karahan, G., Sayar, N. ... Yuluğ, I. (2016). Metastasis suppressor proteins in cutaneous squamous cell carcinoma. Pathology Research And Practice, 212(7), 608-615. https://dx.doi.org/10.1016/j.prp.2015.12.018
dc.identifier.doi10.1016/j.prp.2015.12.018
dc.identifier.endpage615
dc.identifier.issn0344-0338
dc.identifier.issue7
dc.identifier.scopusqualityQ2
dc.identifier.startpage608
dc.identifier.urihttps://dx.doi.org/10.1016/j.prp.2015.12.018
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3634
dc.identifier.volume212
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofPathology Research And Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSkin
dc.subjectSquamous Cell Carcinoma
dc.subjectMetastasis Suppressor Proteins
dc.subjectA-431
dc.subjectHaCaT
dc.titleMetastasis suppressor proteins in cutaneous squamous cell carcinoma
dc.typeArticle

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