Design of dense brush conformation bearing gold nanoparticles as theranostic agent for cancer

Basit Öğe Kaydı
dc.contributor.authorVerimli, Nihan
dc.contributor.authorDemiral, Ayşegül
dc.contributor.authorYılmaz, Hülya
dc.contributor.authorÇulha, Mustafa
dc.contributor.authorErdem, Sultan Sibel
dc.date.accessioned2020-01-29T06:17:05Z
dc.date.available2020-01-29T06:17:05Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractDense brush conformation–bearing theranostic agents are emerging as drug delivery systems due to their higher ability to escape from reticuloendothelial system uptake which prolongs their in vivo circulation time. With the aim of developing dual therapy agent, 13-nm gold nanoparticles’ (AuNPs) surfaces were coated with different amounts of polyethylene glycol (PEG) (SH-PEG-NH2) to obtain dense brush conformation–bearing theranostic agents. Among the 14 different theranostic agent candidates prepared, the one hosting 1819 PEG per particle was selected as the most promising theranostic agent candidate based on structural conformation, stability, size, zeta potential, hemocompatibility, cell inhibition, and cell death pathway towards MCF-7 cell line. To test drug delivery efficiency of the developed PEGylated AuNP and to improve efficacy of the treatment, apoptotic peptide (AP) was covalently conjugated to NH2 terminus of the PEG in various ratios to yield AuNP-AP conjugate. Among the prepared conjugates, the one having 1 nmol of peptide per milliliter of AuNP yielded the most promising results based on the same criteria as employed for PEGylated AuNPs. Besides, incorporation of AP to AuNP returned in superior efficacy of AP since it was possible to achieve 50% cell death with 1000 times less amount of AP alone.
dc.identifier.citationVerimli, N., Demiral, A., Yılmaz, A., Çulha, M. ve Erdem, S. S. (2019). Design of dense brush conformation bearing gold nanoparticles as theranostic agent for cancer. Applied Biochemistry and Biotechnology, 189(3), 709-728. https://doi.org/10.1007/s12010-019-03151-6
dc.identifier.doi10.1007/s12010-019-03151-6
dc.identifier.endpage728
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue3
dc.identifier.scopusqualityQ2
dc.identifier.startpage709
dc.identifier.urihttps://doi.org/10.1007/s12010-019-03151-6.
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4932
dc.identifier.volume189
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectApoptosis
dc.subjectApoptotic Peptide
dc.subjectBreast Cancer
dc.subjectDrug Delivery
dc.subjectGold Nanoparticles
dc.subjectHemocompatibility
dc.subjectPolyethylene Glycol
dc.subjectTheranostic Agent
dc.titleDesign of dense brush conformation bearing gold nanoparticles as theranostic agent for cancer
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
verimli-nihan(2019).pdf
Boyut:
1.75 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu