In vitro myelin formation using embryonic stem cells

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dc.authorid0000-0003-1106-3288
dc.contributor.authorKerman, Bilal Ersen
dc.contributor.authorKim, Hyung-joon
dc.contributor.authorPadmanabhan, Krishnan
dc.contributor.authorMei, Arianna
dc.contributor.authorGeorges, Shereen
dc.contributor.authorJoens, Matthew
dc.contributor.authorFitzpatrick, James
dc.contributor.authorJappelli, Roberto
dc.contributor.authorChandross, Karen
dc.contributor.authorAugust, Paul
dc.contributor.authorGage, Fred
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:35:40Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:35:40Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.description.abstractMyelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.
dc.description.sponsorshipNational Institutes of Health: P30CA014195 National Institutes of Health: P30NS072031en_US
dc.description.sponsorshipNational Institutes of Health: P30NS072031en_US
dc.description.sponsorshipNational Multiple Sclerosis Societyen_US
dc.description.sponsorshipSanofi and Salk Institute Discovery Awarden_US
dc.description.sponsorshipChristopher & Dana Reeve Foundationen_US
dc.description.sponsorshipLeona M. and Harry B. Helmsley Charitable Trusten_US
dc.description.sponsorshipAnnette Merle-Smithen_US
dc.description.sponsorshipCrick-Jacobs Junior Fellowshipen_US
dc.description.sponsorshipCalifornia Institute for Regenerative Medicineen_US
dc.description.sponsorshipWaitt Advanced Biophotonics Centeren_US
dc.description.sponsorshipW.M. Keck Foundationen_US
dc.description.sponsorshipNational Cancer Institute (NCI) P30 Cancer Center Support Granten_US
dc.description.sponsorshipNational Institute of Neurological Disorders and Stroke (NINDS) P30 Neuroscience Center Core Granten_US
dc.identifier.citationKerman, B. E., Kim, H. J., Padmanabhan, K., Mei, A., Georges, S., Joens, M. S. ... Gage, F. (2015). In vitro myelin formation using embryonic stem cells. Development, 142(12), 2213-2225. https://dx.doi.org/10.1242/dev.116517
dc.identifier.doi10.1242/dev.116517
dc.identifier.endpage2225
dc.identifier.issn0950-1991
dc.identifier.issn1477-9129
dc.identifier.issue12
dc.identifier.scopusqualityQ1
dc.identifier.startpage2213
dc.identifier.urihttps://hdl.handle.net/20.500.12511/893
dc.identifier.urihttps://dx.doi.org/10.1242/dev.116517
dc.identifier.volume142
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCompany of Biologists
dc.relation.ispartofDevelopmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAutomated Quantification
dc.subjectEmbryonic Stem Cells
dc.subjectLive Imaging
dc.subjectMyelin
dc.subjectOligodendrocyte
dc.titleIn vitro myelin formation using embryonic stem cells
dc.typeArticle

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