Comparison of S gene mutations in patients with occult and chronic hepatitis B virus infection

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dc.contributor.authorÇakal, Bülent
dc.contributor.authorÇavuş, Bilger
dc.contributor.authorAtasoy, Alp
dc.contributor.authorAltunok, Damla
dc.contributor.authorPoda, Mehveş
dc.contributor.authorBulakçı, Mesut
dc.contributor.authorGülluoğlu, Mine
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorTürker Şener, Leyla
dc.contributor.authorArslan, Aslı Berru
dc.contributor.authorArıkan, Muzaffer
dc.contributor.authorAkyüz, Filiz
dc.date.accessioned2022-07-29T06:14:19Z
dc.date.available2022-07-29T06:14:19Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractBackground and aim: This study aimed to detect mutations in the HBV S gene and evaluate their relationship to occult hepatitis B virus (HBV) infection (OBI). Methods: The study included 32 patients with negative serum HBsAg and HBV DNA who underwent liver biopsy due to different clinical indications defined as the OBI group and 32 patients who underwent liver biopsy due to chronic hepatitis B (CHB) as the comparison group. The HBV S gene region was amplified by Nested PCR, and Sanger sequencing was performed. Results: At least one amino acid (aa) mutation was detected in the major hydrophilic region (MHR) of the HBV S gene in 14/32 (43.75%) of the patients with OBI and 8/32 (25.0%) with CHB. The genotype of all patients with OBI and CHB was HBV/D. Although 9 (28.1%) of the cases with OBI had sub-genotype HBV/D3, none of the patients with CHB had sub-genotype HBV/D3. Unlike patients with CHB, L15*, D33N, Q51P, V63F, L91I, P108S, T115I, P120L, T125M, Q129H, T189I, L216F, P217L mutations were detected in the HBV S gene in OBI cases. Also, P127T aa polymorphism was frequently detected. Mutation frequency in the HBV S gene in the major hydrophilic region (MHR) was higher in patients with OBI with sub-genotypes HBV/D3 and D2 than those with HBV/D1 and those with serotype HBV/ayw3 compared to those with HBV/ayw2 (p < 0.05). Conclusions: Sub-genotypic-specific mutation patterns were seen in the “a” determinant region and T helper cell epitopes of HBsAg, especially in the C-terminus domain; this may be associated with OBI.
dc.identifier.citationÇakal, B., Çavuş, B., Atasoy, A., Altunok, D., Poda, M., Bulakçı, M. ... Akyüz, F. (2022). Comparison of S gene mutations in patients with occult and chronic hepatitis B virus infection. Virus Research, 318. https://doi.org/10.1016/j.virusres.2022.198855
dc.identifier.doi10.1016/j.virusres.2022.198855
dc.identifier.pmid35798213
dc.identifier.scopus2-s2.0-85134302209
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.virusres.2022.198855
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9617
dc.identifier.volume318
dc.identifier.wos000836303100001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorArıkan, Muzaffer
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofVirus Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectOccult HBV Infection
dc.subjectHBV S Gene
dc.subjectImmune Epitopes
dc.subjectMutation
dc.subjectSub-Genotype
dc.titleComparison of S gene mutations in patients with occult and chronic hepatitis B virus infection
dc.typeArticle

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