Lack of association between increased mitochondrial DNA(4977) deletion and ATP levels of sputum cells from chronic obstructive pulmonary disease patients versus healthy smokers

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dc.authorid0000-0002-0060-2859
dc.contributor.authorKarimova, Ayla
dc.contributor.authorMüşteri Oltulu, Yasemin
dc.contributor.authorAzaklı, Hülya
dc.contributor.authorKara, Murat
dc.contributor.authorÜstek, Duran
dc.contributor.authorTutluoğlu, Bülent
dc.contributor.authorOnaran, İlhan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:48Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:48Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı
dc.descriptionWOS: 000397858400027
dc.descriptionPubMed ID: 26713688
dc.description.abstractIn this study we looked at smokers with and without chronic obstructive pulmonary disease (COPD) patients in order to evaluate the incidence of 4977 base pair (bp) mtDNA (mtDNA(4977)) deletion and mtDNA copy number in sputum cells and in peripheral blood leukocytes (PBLs) in relation to mitochondrial function and oxidative stress status. Twenty-five COPD patients who were current smokers, 22 smokers and 23 healthy nonsmokers (for only PBLs studies) participated in this study. The 4977-bp deletion was detected in all examined samples within 40 cyles of PCR amplification, using a quantitative real time PCR. The frequency of the mtDNA(4977) was significantly higher in the sputum cells of patients with COPD compared to smokers without COPD (p<0.0001). This difference was not observed in PBLs. Levels of cellular oxidative stress were significantly higher in the sputum cells of subjects with COPD than in the smoker group. However, mtDNA copy number, mitochondrial membrane potential (Delta Psi m) and cellular ATP levels in PBLs and sputum cells were not significantly different between the studied groups. The Pearson analysis revealed no correlations between the accumulation of mtDNA(4977), and intracellular ATP content and Delta Psi m values of the sputum cells, although there was a positive correlation between the increase in the percentage of deleted mtDNA(4977) and the levels of cellular oxidative stress in COPD patients (r = 0.80, p<0.0001). Our studies may suggest that the accumulation of mtDNA(4977) in the sputum cells of smokers with COPD does not seem to have an important impact on mitochondrial dysfunction in relation to ATP production and Delta Psi m when compared to those of healthy smokers.
dc.description.sponsorshipResearch Fund of Istanbul University [T81/12122006]en_US
dc.description.sponsorshipThere is no conflict of interest among the author. This work was supported by the Research Fund of Istanbul University (project number: T81/12122006).en_US
dc.identifier.citationKarimova, A., Müşteri Oltulu, Y., Azaklı, H., Kara, M., Üstek, D., Tutluoğlu, B. ve Onaran, İ. (2017). Lack of association between increased mitochondrial DNA(4977) deletion and ATP levels of sputum cells from chronic obstructive pulmonary disease patients versus healthy smokers. Mitochondrial DNA Part A, 28(3), 361-369. https://dx.doi.org/10.3109/19401736.2015.1126826
dc.identifier.doi10.3109/19401736.2015.1126826
dc.identifier.endpage369
dc.identifier.issn2470-1394
dc.identifier.issn2470-1408
dc.identifier.issue3
dc.identifier.scopusqualityQ4
dc.identifier.startpage361
dc.identifier.urihttps://dx.doi.org/10.3109/19401736.2015.1126826
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3449
dc.identifier.volume28
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd.
dc.relation.ispartofMitochondrial DNA Part Aen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectATP
dc.subjectCommon Mutation
dc.subjectCOPD
dc.subjectMitochondrial DNA
dc.subjectSmoking
dc.subjectSputum
dc.titleLack of association between increased mitochondrial DNA(4977) deletion and ATP levels of sputum cells from chronic obstructive pulmonary disease patients versus healthy smokers
dc.typeArticle

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