Modulation of the excitability of stellate neurons in the ventral cochlear nucleus of mice by TRPM2 channels

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dc.authorid0000-0003-0352-1947
dc.contributor.authorBal, Ramazan
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorÖnalan Etem, Ebru
dc.contributor.authorEraslan, Ersen
dc.contributor.authorÖzaydın, Seda
dc.date.accessioned2020-09-03T07:51:00Z
dc.date.available2020-09-03T07:51:00Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.description.abstractOxidative stress-induced Ca2+ permeable transient receptor potential melastatin 2 (TRPM2) channels are expressed at high levels in the brain, appear to link neuronal excitability to cellular metabolism, and are involved in the pathogenesis of neurodegenerative disorders. We aimed to study the electrophysiological properties of TRPM2 channels in stellate cells of the mouse ventral cochlear nucleus (VCN) using molecular, immunohistochemical and electrophysiological approaches.In the present study, the real time PCR analysis revealed the presence of the TRPM2 mRNA in the mouse VCN tissue. Cell bodies of stellate cells were moderately labeled with TRPM2 antibodies using immunohistochemical staining.Stellate cells were sensitive to intracellular ADP-ribose (ADPR), a TRPM2 agonist. Upon the application of ADPR, the resting membrane potential of the stellate cells was significantly depolarized, shifting from -61.2 +/- 0.9 mV to-57.0 +/- 0.8 mV (P < 0.001; n = 21), and the firing rate significantly increased (P < 0.001, n = 6). When the pipette solution contained ADPR (300 mu M) and the TRPM2 antagonists flufenamic acid (FFA) (100 mu M), N-(p-amylcinnamoyl) anthranilic acid (ACA) (50 mu M) and 8-bromo-cADP-Ribose (8Br-cADPR) (50 mu M), the membrane potential shifted in a hyperpolarizing direction. ADPR did not significantly change the resting membrane potential and action potential firing rate of stellate cells from TRPM2-/- mice.In conclusion, the results obtained using these molecular, immunohistochemical and electrophysiological approaches reveal the expression of functional TRPM2 channels in stellate neurons of the mouse VCN. TRPM2 might exert a significant modulatory effect on setting the level of resting excitability.
dc.identifier.citationBal, R., Öztürk, G., Önalan Etem, E., Eraslan, E. ve Özaydın, S. (2020). Modulation of the excitability of stellate neurons in the ventral cochlear nucleus of mice by TRPM2 channels. European Journal of Pharmacology, 882. https://dx.doi.org/10.1016/j.ejphar.2020.173163
dc.identifier.doi10.1016/j.ejphar.2020.173163
dc.identifier.issn0014-2999
dc.identifier.issn1879-0712
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1016/j.ejphar.2020.173163
dc.identifier.urihttps://hdl.handle.net/20.500.12511/5767
dc.identifier.volume882
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/109S516
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/110S397
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectStellate Cells
dc.subjectCochlear Nucleus
dc.subjectTRPM2 Channels
dc.subjectTRP Channel Family
dc.titleModulation of the excitability of stellate neurons in the ventral cochlear nucleus of mice by TRPM2 channels
dc.typeArticle

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