Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano

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dc.contributor.authorWudarski, Jakub
dc.contributor.authorSimanov, Daniil
dc.contributor.authorUstyantsev, Kirill
dc.contributor.authorDe Mulder, Katrien
dc.contributor.authorGrelling, Margriet
dc.contributor.authorGrudniewska, Magda
dc.contributor.authorBeltman, Frank
dc.contributor.authorGlazenburg, Lisa
dc.contributor.authorDemircan, Turan
dc.contributor.authorWunderer, Julia
dc.contributor.authorQi, Weihong
dc.contributor.authorVizoso, Dita B.
dc.contributor.authorWeissert, Philipp M.
dc.contributor.authorOlivieri, Daniel
dc.contributor.authorMouton, Stijn
dc.contributor.authorGuryev, Victor
dc.contributor.authorAboobaker, Aziz
dc.contributor.authorScharer, Lukas
dc.contributor.authorLadurner, Peter
dc.contributor.authorBerezikov, Eugene
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:53Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:53Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.descriptionWOS: 000417906400004
dc.descriptionPubMed ID: 29242515
dc.description.abstractRegeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and show its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration using M. lignano as a model organism.
dc.description.sponsorshipEuropean Research Council (ERC Starting Grant "MacModel") [310765]; Russian State Budget [0324-2016-0008]; Biotechnology and Biological Sciences Research Council (BBSRC) [BB/K007564/1]; Austrian Science Fund (FWF) [25404]; Swiss National Science Foundation (SNFS) [3100A0-127503, 31003A-143732]; Russian Foundation for Basic Research (RFBR) [15-04-08003]en_US
dc.description.sponsorshipWe thank H. Clevers for the support on the early stages of the project; E. Cuppen, E. de Bruin, P. van Zon and H. Lunstroo for the help with generating 454 data, and ERIBA sequencing facility for generating Illumina data. This work was supported by the European Research Council (ERC Starting Grant "MacModel", grant no. 310765) to E.B., K.U. was supported by the project 0324-2016-0008 from the Russian State Budget. A. A. was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant no. BB/K007564/1). P.L. was supported by the Austrian Science Fund (FWF, grant no. 25404). L.S. was supported by the Swiss National Science Foundation (SNFS, grant no. 3100A0-127503 and 31003A-143732). The work on annotation of transposable elements was supported by the Russian Foundation for Basic Research (RFBR, grant no. 15-04-08003) to E.B.en_US
dc.identifier.citationWudarski, J., Simanov, D., Ustyantsev, K., De Mulder, K., Grelling, M., Grudniewska, M. ... Berezikov, E. (2017). Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano. Nature Communications, 8. https://dx.doi.org/10.1038/s41467-017-02214-8
dc.identifier.doi10.1038/s41467-017-02214-8
dc.identifier.issn2041-1723
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1038/s41467-017-02214-8
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3479
dc.identifier.volume8
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.ispartofNature Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectEfficient Transgenesis
dc.subjectAnnotated Genome Sequence
dc.subjectRegenerative Flatworm Model
dc.subjectMacrostomum Lignano
dc.titleEfficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano
dc.typeArticle

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