The neuroprotective effects of hypothermia on bilirubin-ınduced neurotoxicity in vitro

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dc.authorid0000-0003-0352-1947
dc.contributor.authorKuter, Nazlı
dc.contributor.authorAyşit-Altuncu, Neşe
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorÖzek, Eren
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:54Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:54Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000433962200010
dc.descriptionPubMed ID: 29510380
dc.description.abstractBackground: In high-risk newborns indirect hyperbilirubinennia can lead to acute bilirubin encephalopathy and kernicterus. Despite the current therapeutic modalities, preventing or reversing the neurotoxicity cannot be achieved in all infants. Objective: To investigate the neuroprotective effects of hypothermia on bilirubin-induced toxicity in primary mouse neuronal cell cultures. Methods: Hippocannpal cell cultures, isolated from newborn mouse brains, were incubated with unconjugated bilirubin (UCB) at 3 days in vitro (DIV) and immediately exposed to varying degrees of hypothermia. Neuronal viability and mitochondrial health were compared between the nornnothernnia (37 degrees C), mild (34 degrees C), moderate (32 degrees C) and severe (29 degrees C) hypothermia groups. Confocal microscopy and fluorescent dyes (propidiunn iodide and JC-1) were used for cell evaluation. To determine the late effects of hypothermia, the cultures were also examined at 7 DIV after returning to normothermic conditions. Results: Induction of any degree of hypothermia increased the neuronal survival after 24 h of UCB treatment, Neuronal death rate and mitochondrial membrane potential loss were lowest in the neurons exposed to moderate hypothermia, We also observed that mild to moderate hypothermia had late protective effects on neuronal cell viability, whereas deep hypothermia did not improve neuronal survival. Conclusions: We conclude that hypothermia reduces the cell death induced by bilirubin toxicity in neuronal cells, Although moderate hypothermia has a better outcome than mild hypothermia, deep hypothermia as low as 29 degrees C has adverse effects on neuronal cell viability.
dc.description.sponsorshipMarmara University Scientific Research Projects Committee, Istanbul, Turkey [SAG-C-TUP-250816-0412]en_US
dc.description.sponsorshipThis study was supported by a grant from the Marmara University Scientific Research Projects Committee, Istanbul, Turkey (SAG-C-TUP-250816-0412).en_US
dc.identifier.citationKuter, N., Ayşit-Altuncu, N., Öztürk, G. ve Özek, E. (2018). The neuroprotective effects of hypothermia on bilirubin-ınduced neurotoxicity in vitro. Neonatology, 113(4), 360-365. https://dx.doi.org/10.1159/000487221
dc.identifier.doi10.1159/000487221
dc.identifier.endpage365
dc.identifier.issn1661-7800
dc.identifier.issn1661-7819
dc.identifier.issue4
dc.identifier.scopusqualityQ1
dc.identifier.startpage360
dc.identifier.urihttps://dx.doi.org/10.1159/000487221
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3483
dc.identifier.volume113
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKarger
dc.relation.ispartofNeonatologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHyperbilirubinemia
dc.subjectNeurotoxicity
dc.subjectHypothermia
dc.titleThe neuroprotective effects of hypothermia on bilirubin-ınduced neurotoxicity in vitro
dc.typeArticle

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