Enhancement of cultured adult motor neuron survival with cold pre-incubation

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dc.authorid0000-0003-0352-1947
dc.contributor.authorBektaş, Serap
dc.contributor.authorÖztürk, Gürkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:02Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:02Z
dc.date.issued2013
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000314016000005
dc.descriptionPubMed ID: 23178188
dc.description.abstractThe aim of the study was to extend the survival of adult spinal motor neurons in serum free culture. Anterior half of the spinal cord was removed from young adult mice and dissociated. Cultured cells attempted to extend neurites within hours of incubation at 37 degrees C and died within 24 h. To prevent this early regenerative activity, thus to decrease the metabolic requirements of the neurons, cultures were transferred to 4 degrees C immediately after they were set and kept there for 3 days. Preparations were then taken to 37 degrees C where they lived up to 8 days. Some neurons continued to extend neurites until the day they died. To understand whether the enhancement of survival involves new protein synthesis, transcription and translation were blocked during cold pre-incubation, which shortened the half life of neurons but not changed the maximum survival period. In conclusion this study has shown that, in the serum-free cultures, the survival of adult spinal motor neurons can be significantly enhanced by cold pre-incubation whose effect seems to depend largely on a reduction in the metabolic activity and less on new protein synthesis.
dc.identifier.citationBektaş, S. ve Öztürk, G. (2013). Enhancement of cultured adult motor neuron survival with cold pre-incubation. Neuroscience Letters, 533(1), 23-27. https://dx.doi.org/10.1016/j.neulet.2012.11.013
dc.identifier.doi10.1016/j.neulet.2012.11.013
dc.identifier.endpage27
dc.identifier.issn0304-3940
dc.identifier.issue1
dc.identifier.scopusqualityQ2
dc.identifier.startpage23
dc.identifier.urihttps://dx.doi.org/10.1016/j.neulet.2012.11.013
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3528
dc.identifier.volume533
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofNeuroscience Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectSpinal Motor Neurons
dc.subjectSerum-Free Culture
dc.subjectHypothermia
dc.subjectSurvival
dc.titleEnhancement of cultured adult motor neuron survival with cold pre-incubation
dc.typeArticle

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