Morphology-dependent immunomodulatory coating of hydroxyapatite/PEO for magnesium-based bone implants

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dc.contributor.authorFarshid, Safoura
dc.contributor.authorKharaziha, Mahshid
dc.contributor.authorSalehi, Hossein
dc.contributor.authorGanjalikhani Hakemi, Mazdak
dc.date.accessioned2023-11-13T07:02:53Z
dc.date.available2023-11-13T07:02:53Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractOne of the most critical issues concerning orthopedic implants is the risk of chronic inflammation, which poses a threat to the bone healing process. Osteo-immunomodulation plays a pivotal role in implant technology by influencing proinflammatory and anti-inflammatory responses, ultimately promoting bone healing. This study aims to investigate the morphology-dependent osteo-immunomodulatory properties of a hydroxyapatite (HA)/plasma electrolytic oxidation (PEO)-coated WE43 alloy. In this context, following the PEO process with various operational parameters (duty cycles of 50-40, 50-20, 70-40%, and frequencies of 0.5, 0.8, and 1 kHz), a layer of HA was applied as the top coating using a straightforward hot-dip process. The results revealed the formation of the PEO layer with distinct morphologies and pore sizes, depending on the operational parameters. Specifically, a uniform PEO coating with small pore sizes (5.2-5.3 ?m) led to the creation of plate-like HA particles, while a random-like HA structure formed on nonuniform surfaces with large pores (7.0-11.1 ?m) of PEO. Moreover, it was observed that the plate-like HA coating exhibited higher adhesion strength than the random one (classified as class 2 vs class 3 based on cross-cut standards). Furthermore, electrochemical impedance spectroscopy (EIS) and polarization studies confirmed a substantial increase in the polarization resistance (680 k?) and total impedance (48?559.6 ?) for the plate-like HA/PEO as compared to the substrate (an increase of 1511-fold and 311-fold, respectively) and the random HA/PEO samples (an increase of 85-fold and 18-fold, respectively). In addition, compared to random HA coatings, there was a significant enhancement in the viability (150% control vs 96% control), proliferation, and differentiation of MG63 cells when exposed to plate-like HA coatings. Moreover, surface morphology and chemistry pronouncedly impacted macrophages' viability, morphology, and phenotype. Notably, plate-like HA coatings resulted in a higher upregulation of BMP-2 and TGF-? than proinflammatory cytokines (IL-6 and M-CSF), indicating a polarization of macrophage type 1 (M1) toward type 2 (M2). In summary, the bilayer HA/PEO coating exhibited remarkable osteo-immunomodulatory activity, making it highly appealing for use in bone implant applications.
dc.identifier.citationFarshid, S., Kharaziha, M., Salehi, H. ve Ganjalikhani Hakemi, M. (2023). Morphology-dependent immunomodulatory coating of hydroxyapatite/PEO for magnesium-based bone implants. ACS Applied Materials & Interfaces, 15(42), 48996-49011. https://dx.doi.org/10.1021/acsami.3c11184
dc.identifier.doi10.1021/acsami.3c11184
dc.identifier.endpage49011
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.issue42
dc.identifier.pmid37831072
dc.identifier.scopus2-s2.0-85175270021
dc.identifier.scopusqualityQ1
dc.identifier.startpage48996
dc.identifier.urihttps://dx.doi.org/10.1021/acsami.3c11184
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11761
dc.identifier.volume15
dc.identifier.wos001090438700001en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGanjalikhani Hakemi, Mazdak
dc.language.isoen
dc.relation.ispartofACS Applied Materials & Interfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHydroxyapatite
dc.subjectImmunomodulation Activity
dc.subjectMagnesium Alloy
dc.subjectOrthopedic Implant
dc.subjectPlate-Like Morphology
dc.titleMorphology-dependent immunomodulatory coating of hydroxyapatite/PEO for magnesium-based bone implants
dc.typeArticle

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