Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo

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dc.authorid0000-0002-9373-0808
dc.contributor.authorGroschup, Bernhard
dc.contributor.authorCalandra, Gian Marco
dc.contributor.authorRaitmayr, Constanze
dc.contributor.authorShrouder, Joshua
dc.contributor.authorLlovera, Gemma
dc.contributor.authorZaki, Asal Ghaffari
dc.contributor.authorBurgstaller, Sandra
dc.contributor.authorBischof, Helmut
dc.contributor.authorEroğlu, Emrah
dc.contributor.authorLiesz, Arthur
dc.contributor.authorMalli, Roland
dc.contributor.authorFilser, Severin
dc.date.accessioned2024-06-28T12:22:58Z
dc.date.available2024-06-28T12:22:58Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractNeuronal activity is accompanied by a net outflow of potassium ions (K+) from the intra- to the extracellular space. While extracellular [K+] changes during neuronal activity are well characterized, intracellular dynamics have been less well investigated due to lack of respective probes. In the current study we characterized the FRET-based K+ biosensor lc-LysM GEPII 1.0 for its capacity to measure intracellular [K+] changes in primary cultured neurons and in mouse cortical neurons in vivo. We found that lc-LysM GEPII 1.0 can resolve neuronal [K+] decreases in vitro during seizure-like and intense optogenetically evoked activity. [K+] changes during single action potentials could not be recorded. We confirmed these findings in vivo by expressing lc-LysM GEPII 1.0 in mouse cortical neurons and performing 2-photon fluorescence lifetime imaging. We observed an increase in the fluorescence lifetime of lc-LysM GEPII 1.0 during periinfarct depolarizations, which indicates a decrease in intracellular neuronal [K+]. Our findings suggest that lc-LysM GEPII 1.0 can be used to measure large changes in [K+] in neurons in vitro and in vivo but requires optimization to resolve smaller changes as observed during single action potentials.
dc.description.sponsorshipGerman Research Foundation (DFG)
dc.description.sponsorshipAustrian Science Fund (FWF)
dc.identifier.citationGroschup, B., Calandra, G. M., Raitmayr, C., Shrouder, J., Llovera, G., Zaki, A. G. ... Filser, S. (2024). Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo. Scientific Reports, 14(1). http://dx.doi.org/10.1038/s41598-024-62993-1
dc.identifier.doi10.1038/s41598-024-62993-1
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid38877089
dc.identifier.scopus2-s2.0-85196039131
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1038/s41598-024-62993-1
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12673
dc.identifier.volume14
dc.identifier.wosWOS:001294005000011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorZaki, Asal Ghaffari
dc.institutionauthorEroğlu, Emrah
dc.language.isoen
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAction Potentials
dc.subjectAnimals
dc.subjectBiosensing Techniques
dc.subjectCells
dc.subjectCultured
dc.subjectFluorescence Resonance Energy Transfer
dc.subjectMice
dc.subjectNeurons
dc.subjectOptogenetics
dc.subjectPotassium
dc.titleProbing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo
dc.typeArticle

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