Panoramic dental X-ray exposure leads to oxidative stress, inflammation and apoptosis-mediated developmental defects in zebrafish embryos

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dc.authorid0000-0002-6274-801X
dc.contributor.authorKaragöz, Atakan
dc.contributor.authorBeler, Merih
dc.contributor.authorAltun, Büşra Dilara
dc.contributor.authorÜnal, İsmail
dc.contributor.authorCansız, Derya
dc.contributor.authorGündüz, Hüseyin
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorEmekli Alturfan, Ebru
dc.contributor.authorErçalık Yalçınkaya, Şebnem
dc.date.accessioned2023-11-10T07:42:53Z
dc.date.available2023-11-10T07:42:53Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractPanoramic x-ray units are widely used in dental radiodiagnostics. Patients are exposed to relatively low radiation doses with panoramic imaging, but considering lifetime frequency of exposure, even a small risk can have serious health consequences. Our aim was to assess the effects of panoramic x-rays at two different exposure times on developing zebrafish embryos, focusing on oxidative stress, inflammation, apoptotic pathways, and development. Zebrafish embryos were divided into three groups: control, standard panoramic (SPE, 5.5 s exposure time) and pedodontic panoramic x-ray group (PPE, 4.8 s exposure time). Optically stimulated luminescence dosimeters were used to measure absorbed doses. Mean radiation doses for SPE and PPE were 7.83 mSv and 5.83 mSv respectively. At the end of 96 h post-fertilization, lipid peroxidation (LPO), nitric oxide (NO), reduced glutathione (GSH), glutathione S-transferase and superoxide dismutase were measured in the embryos. Expressions of genes related with inflammation (tnf?, il6, ill15, il21), immunoregulation (ifng) and apoptosis (p53, bax, casp2, casp3, casp8) were determined by RT-PCR. Even at reduced doses at high-speed mode, developmental toxicity was observed in both groups as evidenced by decreased pigmentation, yolk sac oedema, and spinal curvature. While deterioration of oxidant-antioxidant balance, suppression of immune response, induction of inflammation and apoptosis were observed through increased LPO, NO, decreased GSH, ifng, and increased expressions of genes related with inflammation and apoptosis, these effects were more pronounced in the SPE group. These results demonstrate the influence of exposure time and indicate the need for further consideration of optimal panoramic modes from a radiation-induced damage perspective.
dc.identifier.citationKaragöz, A., Beler, M., Altun, B. D., Ünal, İ., Cansız, D., Gündüz, H. ... Erçalık Yalçınkaya, Ş. (2023). Panoramic dental X-ray exposure leads to oxidative stress, inflammation and apoptosis-mediated developmental defects in zebrafish embryos. Journal of Stomatology, Oral and Maxillofacial Surgery, 124(6). https://dx.doi.org/10.1016/j.jormas.2023.101661
dc.identifier.doi10.1016/j.jormas.2023.101661
dc.identifier.issn2468-7855
dc.identifier.issn2468-8509
dc.identifier.issue6
dc.identifier.pmid37866507
dc.identifier.scopus2-s2.0-85175307764
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.1016/j.jormas.2023.101661
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11756
dc.identifier.volume124
dc.identifier.wos001122519000056en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherElsevier Masson s.r.l.
dc.relation.ispartofJournal of Stomatology, Oral and Maxillofacial Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectApoptosis
dc.subjectDevelopment
dc.subjectDiagnostic X-Rays
dc.subjectPanoramic
dc.subjectZebrafish Embryos
dc.titlePanoramic dental X-ray exposure leads to oxidative stress, inflammation and apoptosis-mediated developmental defects in zebrafish embryos
dc.typeArticle

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