A pooled RT-PCR testing strategy for more efficient Covid-19 pandemic management

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dc.authorid0000-0001-6930-7148
dc.authorid0000-0002-2259-4503
dc.authorid0000-0003-0352-1947
dc.authorid0000-0002-3386-1734
dc.contributor.authorAyaz, Akif
dc.contributor.authorÖztürk Demir, Aslı Güner
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorKoçak, Mehmet
dc.date.accessioned2022-01-04T06:04:54Z
dc.date.available2022-01-04T06:04:54Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik ve Tıp Bilişimi Ana Bilim Dalı
dc.description.abstractObjectives: RT-PCR testing is indispensable in Covid-19 pandemic management. With the spread of the pandemic with emerging variants, the RT-PCR capacity is overburdened and new strategies and capabilities need to be established. One option is the pooled RT-PCR testing. Design: We conducted an experiment with various mixtures of Covid-19 samples known to be negative and positive, and investigated the impact of pool size and mixture level on final cycle threshold (CT)- measures. More specifically, 5, 10, and 20 Covid-19 negative samples are combined with 1, 2 or 3 low-CT or high-CT Covid-19 positive samples. Results: We have shown that average input CT and number of high and low CT samples in the pool are the main drivers of the final CT assessment, making the detectability easier. Pool size was not significantly associated with Final CT albeit suggestive. Conclusions: We conclude that pooled RT-PCR testing strategy does not reduce the sensitivity of RT-PCR and thus provides a practical way to expand the RT-PCR screening capacity in pandemic management. The pool size not being significnat, we recommend that the pool size of 20 would be a practical level reducing the time to obtain the results and the cost of RT-PCR testing needs.
dc.identifier.citationAyaz, A., Öztürk Demir, A. G., Öztürk, G. ve Koçak, M. (2022). A pooled RT-PCR testing strategy for more efficient Covid-19 pandemic management. International Journal of Infectious Diseases, 116(1-6). https://doi.org/10.1016/j.ijid.2021.12.328
dc.identifier.doi10.1016/j.ijid.2021.12.328
dc.identifier.endpage6
dc.identifier.issn1878-3511
dc.identifier.issn1201-9712
dc.identifier.pmid34922006
dc.identifier.scopus2-s2.0-85122641236
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.ijid.2021.12.328
dc.identifier.urihttps://hdl.handle.net/20.500.12511/8750
dc.identifier.volume116
dc.identifier.wos000789660900001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCovid-19 Testing
dc.subjectCycle Threshold
dc.subjectPandemic Management
dc.subjectPooled RT-PCR
dc.subjectVirus Screening
dc.titleA pooled RT-PCR testing strategy for more efficient Covid-19 pandemic management
dc.typeArticle

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