Radiation type- and dose-specific transcriptional responses across healthy and diseased mammalian tissues

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dc.authorid0000-0002-2865-0414
dc.contributor.authorSagkrioti, Eftychia
dc.contributor.authorBiz, Gökay Mehmet
dc.contributor.authorTakan, Işıl
dc.contributor.authorAsfa, Seyedehsadaf
dc.contributor.authorNikitaki, Zacharenia
dc.contributor.authorZanni, Vassiliki
dc.contributor.authorKars, Rümeysa Hanife
dc.contributor.authorHellweg, Christine E.
dc.contributor.authorAzzam, Edouard I.
dc.contributor.authorLogotheti, Stella
dc.contributor.authorPavlopoulou, Athanasia
dc.contributor.authorGeorgakilas, Alexandros G.
dc.date.accessioned2022-12-22T09:07:55Z
dc.date.available2022-12-22T09:07:55Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractIonizing radiation (IR) is a genuine genotoxic agent and a major modality in cancer treatment. IR disrupts DNA sequences and exerts mutagenic and/or cytotoxic properties that not only alter critical cellular functions but also impact tissues proximal and distal to the irradiated site. Unveiling the molecular events governing the diverse effects of IR at the cellular and organismal levels is relevant for both radiotherapy and radiation protection. Herein, we address changes in the expression of mammalian genes induced after the exposure of a wide range of tissues to various radiation types with distinct biophysical characteristics. First, we constructed a publicly available database, termed RadBioBase, which will be updated at regular intervals. RadBioBase includes comprehensive transcriptomes of mammalian cells across healthy and diseased tissues that respond to a range of radiation types and doses. Pertinent information was derived from a hybrid analysis based on stringent literature mining and transcriptomic studies. An integrative bioinformatics methodology, including functional enrichment analysis and machine learning techniques, was employed to unveil the characteristic biological pathways related to specific radiation types and their association with various diseases. We found that the effects of high linear energy transfer (LET) radiation on cell transcriptomes significantly differ from those caused by low LET and are consistent with immunomodulation, inflammation, oxidative stress responses and cell death. The transcriptome changes also depend on the dose since low doses up to 0.5 Gy are related with cytokine cascades, while higher doses with ROS metabolism. We additionally identified distinct gene signatures for different types of radiation. Overall, our data suggest that different radiation types and doses can trigger distinct trajectories of cell-intrinsic and cell-extrinsic pathways that hold promise to be manipulated toward improving radiotherapy efficiency and reducing systemic radiotoxicities.
dc.identifier.citationSagkrioti, E., Biz, G. M., Takan, I., Asfa, S., Nikitaki, Z., Zanni, V. ... Georgakilas, A. G. (2022). Radiation type- and dose-specific transcriptional responses across healthy and diseased mammalian tissues. Antioxidants, 11(11). https://doi.org/10.3390/antiox11112286
dc.identifier.doi10.3390/antiox11112286
dc.identifier.issn2076-3921
dc.identifier.issue11
dc.identifier.pmid36421472
dc.identifier.scopus2-s2.0-85149476746
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/antiox11112286
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10165
dc.identifier.volume11
dc.identifier.wos000894912400001en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKars, Rümeysa Hanife
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofAntioxidantsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBioinformatics
dc.subjectGene Signature
dc.subjectOxidative Stress
dc.subjectRadiation Response
dc.subjectRadiobiology Database
dc.subjectTranscriptomics
dc.titleRadiation type- and dose-specific transcriptional responses across healthy and diseased mammalian tissues
dc.typeArticle

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