Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder

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dc.authorid0000-0001-9283-582X
dc.contributor.authorGümüştaş, Funda
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorGümüştaş, Seyit Ali
dc.contributor.authorBatmaz, Ahmet Güray
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorAkkaya, Şeyda
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:56:13Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:56:13Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.descriptionWOS: 000408391700009
dc.descriptionPubMed ID: 27837176
dc.description.abstractPurpose: Methylphenidate (MPH) derivative drugs are used because of psychostimulants effects on attention-deficit hyperactivity disorder in children and adults. As far as we know, toxic or anti-proliferative effects of MPH against cartilage tissue were not studied in the literature. The present study was carried out to investigate the possible effects of MPH on the proliferation, viability and differentiation of primary human chondrocytes, in vitro. Methods: Monolayer primary chondrocyte cultures were prepared using osteochondral tissue obtained from patients who underwent a total knee prosthesis operation. Stock solution of MPH was prepared and aliquots having 1-1000 mu M concentrations of the drug was composed. These solutions were applied to the wells containing cultured chondrocyte samples within the well plates. Control groups were composed of pure chondrocyte culture and no solution was added into them. All groups were evaluated at 24, 48 and 72 h in order to determine the possible negative effects of the drug on the chondrocytes. The data were evaluated by Tukey's honestly significantly different test following analysis of variance. Results: In the group where MPH was applied, it was found that viability, proliferation and stage-specific embryonic antigen-1 protein expression were decreased in comparison to the control group. Conclusions: It was emphasized that clinicians should not disregard the fact that this drug might suppress chondrocyte cell proliferation and chondrogenic differentiation.
dc.identifier.citationGümüştaş, F., Yılmaz, İ., Yaşar Şirin, D., Gümüştaş, S. A., Batmaz, A. G., İşyar, M. ... Mahiroğulları, M. (2017). Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder. Human & Experimental Toxicology, 36(9), 981-992. https://dx.doi.org/10.1177/0960327116678294
dc.identifier.doi10.1177/0960327116678294
dc.identifier.endpage992
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue9
dc.identifier.scopusqualityQ2
dc.identifier.startpage981
dc.identifier.urihttps://dx.doi.org/10.1177/0960327116678294
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2623
dc.identifier.volume36
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltd
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectADHD
dc.subjectChondrotoxicity
dc.subjectMethylphenidate
dc.subjectProliferation
dc.subjectSSEA-1
dc.titleChondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder
dc.typeArticle

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