Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

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dc.authorid0000-0003-3302-5004
dc.contributor.authorAltun, Ayşe
dc.contributor.authorErsoy Çanillio?lu, Yasemin
dc.contributor.authorÜnsal, Evrim
dc.contributor.authorKarabulut, Seda
dc.contributor.authorHürda?, Canan
dc.date.accessioned2021-05-21T07:53:39Z
dc.date.available2021-05-21T07:53:39Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractSpermatogenesis is a process that involves cellular and biochemical processes. The purpose of this study is to understand the role of tight junctions in spermatogenesis by evaluating Zonula occludens-1 (ZO-1) protein levels of cells from the testicular sperm extraction (TESE) biopsies in patients with azoospermia. The study included 40 infertile men with azoospermia. They were divided into two groups, as spermatozoa positive (n=20) and spermatozoa negative (n=20). Testis tissues were examined morphologically, immunohistochemically and ultrastructurally by using transmission electron microscopy (TEM). The structure of seminiferous tubules was deteriorated, degenerated and hyalinized in spermatozoa negative group. ZO-1 expression level was normal in blood-testis barrier of spermatozoa positive group in comparing to lateral surfaces of Sertoli cells spermatozoa negative group. The ultrastructural results were also supported with light microscopy findings. These findings suggest that decrease of the ZO-1 protein expression level in Sertoli cells might be one of the factors involved in the impairment of spermatogenesis in spermatozoa negative group. Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.
dc.identifier.citationAltun, A., Ersoy Çanillio?lu, Y., Ünsal, E., Karabulut, S. ve Hürda?, C. (2021). Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. Journal of Experimental and Clinical Medicine (Turkey), 38(3), 272-276. https://dx.doi.org/10.52142/omujecm.38.3.12
dc.identifier.doi10.52142/omujecm.38.3.12
dc.identifier.endpage276
dc.identifier.issn1309-4483
dc.identifier.issue3
dc.identifier.scopusqualityQ4
dc.identifier.startpage272
dc.identifier.urihttps://dx.doi.org/10.52142/omujecm.38.3.12
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6873
dc.identifier.volume38
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherOndokuz Mayıs Üniversitesi
dc.relation.ispartofJournal of Experimental and Clinical Medicine (Turkey)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAzoospermia
dc.subjectMale Infertility
dc.subjectTESE
dc.subjectTight Junction’s Protein ZO-1
dc.titleUltrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases
dc.typeArticle

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