Investigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell cultures

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dc.authorid0000-0001-5488-2262
dc.contributor.authorAydın, İrfan
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2022-06-06T06:43:42Z
dc.date.available2022-06-06T06:43:42Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.description.abstractAIM: To investigate the supplementation of alpha-lipoic acid (ALA) at the molecular level to determine its effect on primary cell cultures prepared from human intervertebral disc (IVD) tissue in an in vitro environment. MATERIAL and METHODS: Human primary cell cultures were prepared from IVD tissue resected during surgery. While cell cultures without ALA supplementation formed the control group, those with ALA supplementation formed the study group. All cell groups were stained using acridine orange/propidium iodide (AO/PI), and the incidence of apoptotic cell death was determined under a fluorescent microscope. Cell surface morphology and extracellular matrix (ECM) structures were evaluated under an invert light microscope. Simultaneously, cell proliferation was evaluated by MTT–ELISA analysis, and the expressions of chondroadherin (CHAD), cartilage oligomeric protein (COMP), interleukin-1 beta (IL-1?), and matrix metalloproteinase (MMP)-7 and-19, which are genes associated with ECM regulation, were tested using qRT–PCR. The data obtained were evaluated statistically using Tukey’s honestly significant difference (HSD) test after analysis of variance (ANOVA) was performed. The alpha significance value was accepted as <.05. RESULTS: Compared to the cells in the control group, it was observed that both proliferation was suppressed and ECM structures deteriorated in the cells in the study group. CONCLUSION: Also, it was reported that the all-gene expression levels changed. ALA supplementation can negatively affect human IVD primary cell cultures in an in vitro environment.
dc.identifier.citationAydın, İ., Yaşar Şirin, D., Karaarslan, N. ve Özbek, H. (2022). Investigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell cultures. Turkish Neurosurgery, 32(3), 420-429. https://doi.org/10.5137/1019-5149.JTN.34917-21.3
dc.identifier.doi10.5137/1019-5149.JTN.34917-21.3
dc.identifier.endpage429
dc.identifier.issn1019-5149
dc.identifier.issue3
dc.identifier.pmid35147963
dc.identifier.scopus2-s2.0-85130493382
dc.identifier.scopusqualityQ3
dc.identifier.startpage420
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.34917-21.3
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9503
dc.identifier.volume32
dc.identifier.wos000804648400012en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, İrfan
dc.language.isoen
dc.publisherTurkish Neurosurgical Society
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAlpha-Lipoic Acid
dc.subjectCartilage Oligomeric Matrix Protein
dc.subjectChondroadherin Gene
dc.subjectInterleukin-1 Beta
dc.subjectIntervertebral Disc
dc.subjectMatrix Metalloproteinase
dc.titleInvestigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell cultures
dc.typeArticle

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