In 2D and 3D cell culture models, effects of endothelial cells on E-cadherin / ?-catenin expression levels and spheroid sizes in ishikawa cells
| dc.authorid | 0000-0002-0885-3417 | |
| dc.authorid | 0000-0003-1526-2065 | |
| dc.authorid | 0000-0001-7933-4262 | |
| dc.authorid | 0000-0002-7059-1884 | |
| dc.contributor.author | Kalender, Mervenur | |
| dc.contributor.author | Bülbül, Muhammet Volkan | |
| dc.contributor.author | Kolbaşı, Bircan | |
| dc.contributor.author | Keskin, İlknur | |
| dc.date.accessioned | 2022-02-25T06:02:26Z | |
| dc.date.available | 2022-02-25T06:02:26Z | |
| dc.date.issued | 2022 | |
| dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı | |
| dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | |
| dc.description.abstract | Increasing evidence shows that three dimensional cell culture models better reflect the in vivo tumor microenvironment than two dimensional cell culture models. Co-culture models are ideal cell culture models for understanding the communication between cells and the in vivo microenvironment. Changes in expression levels of E-cadherin are closely related to cancer metastasis and progression. ?-catenin mediates cell adhesion of E-cadherin. Endothelial cells are stromal cell components in the tumor microenvironment. It is known that there is little or no expression of E-cadherin in endothelial cells. Methods: In our study, both two-dimensional and three dimensional mono-culture and co-culture models were created using Huvec and Ishikawa cells (endometrial cancer cell lines) to better reflect cell interactions. Spheroids were followed for three days in the three-dimensional cell culture model. E-cadherin and ?-catenin expression levels of two-dimensional and three dimensional mono-culture and co-culture models were measured by immunofluorescence staining. Spheroid images were recorded using a Z-stack. Intensity measurements in both two-dimensional and three-dimensional mono-culture and co-culture models were made using the Image J software. Study groups were evaluated by one-way analysis of variance (One-Way ANOVA). Values of p <0.05 were considered statistically significant. Results: The size of the co-culture spheroids was recorded significantly larger than the mono-culture spheroids (p <0.0001). In two (p = 0.0175) and three dimensional models (p <0.0001), expression levels of E-cadherin in the mono-culture of Ishikawa cells were recorded significantly higher than in Huvec and co-culture cells. Likewise, while the expression levels of ?-catenin were higher in the mono-culture of Ishikawa cells in two-dimensional models (p <0.05), no significant difference was observed in three dimensional models (p> 0.05). Conclusion: In summary, it has been noted that the expression levels of E-cadherin are significantly reduced in co-cultures of Ishikawa cells with Huvec cells in both two and three dimensions . These results support the idea that endothelial cells may cause changes in endometrial cancer progression by suppressing E-cadherin expression in Ishikawa cells. | |
| dc.identifier.citation | Kalender, M., Bülbül, M. V., Kolbaşı, B. ve Keskin, İ. (2022). In 2D and 3D cell culture models, effects of endothelial cells on E-cadherin / ?-catenin expression levels and spheroid sizes in ishikawa cells. Asian Pacific Journal of Cancer Prevention, 23(1), 39-51. https://doi.org/10.31557/APJCP.2022.23.1.39 | |
| dc.identifier.doi | 10.31557/APJCP.2022.23.1.39 | |
| dc.identifier.endpage | 51 | |
| dc.identifier.issn | 1513-7368 | |
| dc.identifier.issue | 1 | |
| dc.identifier.pmid | 35092370 | |
| dc.identifier.scopus | 2-s2.0-85123815487 | |
| dc.identifier.scopusquality | Q3 | |
| dc.identifier.startpage | 39 | |
| dc.identifier.uri | https://doi.org/10.31557/APJCP.2022.23.1.39 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12511/9000 | |
| dc.identifier.volume | 23 | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.institutionauthor | Kalender, Mervenur | |
| dc.institutionauthor | Bülbül, Muhammet Volkan | |
| dc.institutionauthor | Kolbaşı, Bircan | |
| dc.institutionauthor | Keskin, İlknur | |
| dc.language.iso | en | |
| dc.publisher | Asian Pacific Organization for Cancer Prevention | |
| dc.relation.ispartof | Asian Pacific Journal of Cancer Prevention | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | Attribution-NonCommercial 4.0 International | * |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | * |
| dc.subject | Co-culture | |
| dc.subject | E-cadherin | |
| dc.subject | Immunofluorescence | |
| dc.subject | Spheroid | |
| dc.subject | ?-catenin | |
| dc.title | In 2D and 3D cell culture models, effects of endothelial cells on E-cadherin / ?-catenin expression levels and spheroid sizes in ishikawa cells | |
| dc.type | Article |
