Therapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model

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dc.contributor.authorŞerefoğlu, Ege Can
dc.contributor.authorKolbaşı, Bircan
dc.contributor.authorBülbül, Muhammet Volkan
dc.contributor.authorKarabulut, Seda
dc.contributor.authorÇakıcı, Çağrı
dc.contributor.authorGündoğdu Özdemir, Reyhan Zeynep
dc.contributor.authorKeskin, İlknur
dc.date.accessioned2025-07-10T08:52:24Z
dc.date.available2025-07-10T08:52:24Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractPurpose: This study aimed to examine the therapeutic effects of injection of conditioned medium of adipose-derived mesenchymal stem cells (ADMSC-CM) in a surgically created varicocele model in comparison with varicocelectomy. Materials and Methods: Twenty-eight male Wistar Albino rats were randomly divided into four groups: sham group, varicocele group, varicocelectomy group, and ADMSC-CM injection group. Sperm parameters were analyzed in samples taken from the epididymis after treatment. Malondialdehyde and superoxide dismutase (SOD) levels in blood samples were examined by biochemical analysis. The testicular tissues were stained with hematoxylin-eosin for histological examination (Johnsen’s Score). Additionally, Western Blot analyzes were performed to detect Claudin-11 levels, the functional protein of the blood-testis barrier, in testicular tissues. Results: Varicocelectomy and ADMSC-CM treatments significantly improved mean sperm parameters (concentration, progressive motility, motility, normal sperm morphology) (p≤0.05 for all). Both treatment groups had increased SOD levels along with a decrease in malondialdehyde levels (p≤0.05 for all). No significant difference was observed between the ADMSC-CM group and the varicocelectomy group in preserving normal testicular histology according to Johnsen’s Score (p=0.114). Levels of Claudin-11 were significantly higher in the varicocelectomy and ADMSC-CM groups compared to the varicocele group (p≤0.05 for all). Conclusions: The therapeutic effects of ADMSC-CM in varicocele model may involve secretion of anti-inflammatory and regenerative factors from ADMSC. ADMSC-CM injection appears to be a promising new strategy in the treatment of varicocele.
dc.description.sponsorshipIstanbul Medipol University Scientific Research Activities Commission Fund
dc.identifier.citationŞerefoğlu, E. C., Kolbaşı, B., Bülbül, M. V., Karabulut, S., Çakıcı, Ç., Gündoğdu Özdemir, R. Z. ... Keskin, İ. (2025). Therapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model. World Journal of Men's Health, 43(2), 437-447. http://dx.doi.org/10.5534/wjmh.240059
dc.identifier.doi10.5534/wjmh.240059
dc.identifier.endpage447
dc.identifier.issn2287-4208
dc.identifier.issn2287-4690
dc.identifier.issue2
dc.identifier.pmid39344116
dc.identifier.scopus2-s2.0-105001310821
dc.identifier.scopusqualityQ1
dc.identifier.startpage437
dc.identifier.urihttp://dx.doi.org/10.5534/wjmh.240059
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13010
dc.identifier.volume43
dc.identifier.wosWOS:001321200800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞerefoğlu, Ege Can
dc.institutionauthorKolbaşı, Bircan
dc.institutionauthorBülbül, Muhammet Volkan
dc.institutionauthorKarabulut, Seda
dc.institutionauthorÇakıcı, Çağrı
dc.institutionauthorKeskin, İlknur
dc.institutionauthorid0000-0002-2530-7012
dc.institutionauthorid0000-0001-7933-4262
dc.institutionauthorid0000-0003-1526-2065
dc.institutionauthorid0000-0003-3302-5004
dc.institutionauthorid0000-0002-8662-5284
dc.institutionauthorid0000-0002-7059-1884
dc.language.isoen
dc.relation.ecinfo:eu-repo/grantAgreement/EC/FP7/BAP22-35
dc.relation.ispartofWorld Journal of Men's Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectClaudins
dc.subjectCulture Media
dc.subjectConditioned
dc.subjectInfertility
dc.subjectMesenchymal Stem Cells
dc.subjectVaricocele
dc.titleTherapeutic effects of mesenchymal stem cell conditioned medium in rat varicocele model
dc.typeArticle

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