Cellular immunity to nucleoproteins (np) of crimean-congo hemorrhagic fever virus (cchfv) and hazara virus (hazv)

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dc.contributor.authorKalkan Yazıcı, Merve
dc.contributor.authorKaraaslan, Elif
dc.contributor.authorGüler Çetin, Nesibe Selma
dc.contributor.authorDoymaz, Mehmet Ziya
dc.date.accessioned2025-06-20T08:49:27Z
dc.date.available2025-06-20T08:49:27Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractCrimean-Congo Hemorrhagic Fever Virus (CCHFV) is a globally significant vector-borne pathogen with no internationally-licensed preventative and therapeutic interventions. Hazara virus (HAZV), on the other hand, a related Orthonairovirus, has not been reported as a human pathogen. HAZV has been proposed as a surrogate model for studying CCHFV, bisosafety level 4 (BSL-4) agent. Previously, we investigated the humoral immune responses between NPs of these viruses and in this study, we extended the scrutiny to cellular immune responses elicited by NPs of CCHFV and HAZV. Here, mice were immunized with recombinant CCHFV NP and HAZV NP to evaluate the correlates of cell-mediated immunity (CMI). Delayed-type hypersensitivity (DTH) responses were assessed by challenging immunized mice with CCHFV-rNP or HAZV-rNP on the footpad and lymphocyte proliferation assays (LPAs) were performed by stimulating splenocytes in vitro with CCHFV-rNP or HAZV-rNP to compare cellular immune responses. In all test groups, strong DTH and LPA responses were detected against homologous and heterologous challenging antigens. To assess the cytokine response, an RT-qPCR -specific for cytokine mRNAs was utilized. Interestingly, CCHFV NP stimulated groups exhibited a significantly elevated mRNA level of interleukin 17 A (IL-17) compared to HAZV NP, indicating a notable difference in immune responses. This study presents comparison between CMI elicited by NPs of CCHFV and HAZV and contributes to the understanding of a highly pathogenic virus, particularly in the context of the declaration of CCHFV by World Health Organization’s (WHO) as a major viral threat to the world.
dc.description.sponsorshipScientific Research Unit of Bezmialem Vakif University
dc.identifier.citationKalkan Yazıcı, M., Karaaslan, E., Güler Çetin, N. S. ve Doymaz, M. Z. (2024). Cellular immunity to nucleoproteins (np) of crimean-congo hemorrhagic fever virus (cchfv) and hazara virus (hazv). Medical Microbiology and Immunology, 213(1). http://dx.doi.org/10.1007/s00430-024-00802-2
dc.identifier.doi10.1007/s00430-024-00802-2
dc.identifier.issn0300-8584
dc.identifier.issn1432-1831
dc.identifier.issue1
dc.identifier.pmid39320473
dc.identifier.scopus2-s2.0-85204899499
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1007/s00430-024-00802-2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12979
dc.identifier.volume213
dc.identifier.wosWOS:001319589500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKalkan Yazıcı, Merve
dc.institutionauthorGüler Çetin, Nesibe Selma
dc.institutionauthorDoymaz, Mehmet Ziya
dc.institutionauthorid0000-0002-3893-1084
dc.institutionauthorid0000-0002-3598-9088
dc.institutionauthorid0000-0003-2066-0252
dc.language.isoen
dc.relation.ecinfo:eu-repo/grantAgreement/EC/FP7/6.2017/27
dc.relation.ecinfo:eu-repo/grantAgreement/EC/FP7/4.2019/12
dc.relation.ispartofMedical Microbiology and Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntigenic Similarity
dc.subjectCCHFV
dc.subjectCellular Immune Response
dc.subjectHAZV
dc.subjectNucleocapsid Protein
dc.titleCellular immunity to nucleoproteins (np) of crimean-congo hemorrhagic fever virus (cchfv) and hazara virus (hazv)
dc.typeArticle

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