The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage

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dc.authorid0000-0002-4899-9146
dc.contributor.authorÖzcan, Pınar
dc.contributor.authorTakmaz, Taha
dc.contributor.authorTok, Olgu Enis
dc.contributor.authorİşlek, Sevde
dc.contributor.authorYiğit, Esra Nur
dc.contributor.authorFıçıcıoğlu, Cem
dc.date.accessioned2020-07-13T10:16:50Z
dc.date.available2020-07-13T10:16:50Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractPurpose We evaluated the protective effect of PRP on ovarian function in female rats with cyclophosphamide (Cy)-induced ovarian damage. Methods Thirty-two adult female Sprague-Dawley rats were randomly divided into four groups. Group 1 (control-sodium chloride 0.9%; 1 mL/kg, single-dose ip injection), group 2 (Cy); 75 mg/kg, single-dose ip injection and sodium chloride 0.9% (1 mL/kg, single-dose ip injection), group 3 Cy plus PRP, Cy (75 mg/kg, single-dose and PRP (200 mu l, single-dose) ip injection), group 4 (PRP, 200 mu l, single-dose ip injection). Primordial, antral, and atretic follicle counts; serum anti-Mullerian hormone (AMH) levels; AMH-positive granulosa cells; and gene expression analysis of Ddx4 were assessed. Results Serum AMH levels were significantly lower in group 2 compared to groups 1, 3, and 4 (p < 0.01, p < 0.01, and p = 0.04, respectively). A significant difference was found in the primordial, primary, secondary, antral, and atretic follicle counts between all groups (p < 0.01). There was a statistically significant difference in AMH-positive staining primary, secondary, and antral follicles count between the groups (p < 0.01). There was a statistically significant difference in primary, secondary, and antral AMH positive staining follicle intensity score between the groups (p < 0.01). Ddx4 expression in group 4 was highest compared to other groups. Conclusion Our study may provide evidence that PRP could protect ovarian function against ovarian damage induced by Cy. It could lead to improved primordial, primary, secondary, and antral follicle numbers.
dc.identifier.citationÖzcan, P., Takmaz, T., Tok, O. E., İşlek, S., Yiğit, E. N. ve Fıçıcıoğlu, C. (2020). The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage. Journal of Assisted Reproduction and Genetics, 37(4), 865-873. https://dx.doi.org/10.1007/s10815-020-01689-7
dc.identifier.doi10.1007/s10815-020-01689-7
dc.identifier.endpage873
dc.identifier.issn1058-0468
dc.identifier.issn1573-7330
dc.identifier.issue4
dc.identifier.scopusqualityQ1
dc.identifier.startpage865
dc.identifier.urihttps://dx.doi.org/10.1007/s10815-020-01689-7
dc.identifier.urihttps://hdl.handle.net/20.500.12511/5467
dc.identifier.volume37
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectFollicle
dc.subjectOvarian Failure
dc.subjectOvary
dc.subjectPlatelet-Rich Plasma
dc.subjectStereology
dc.titleThe protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage
dc.typeArticle

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