Development of molecular-based screening test for hepatitis b virus in human plasma samples

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dc.contributor.authorAltın, Kevser Hanne
dc.contributor.authorAgel, Esra
dc.date.accessioned2025-10-23T06:22:53Z
dc.date.available2025-10-23T06:22:53Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractDespite regular administration of hepatitis B virus (HBV) vaccine in several countries, the mortality rate associated with HBV remains significant. The antiviral medications available for the treatment of HBV infection do not suffice for the serious complications related to chronic HBV infection. Additionally, the serological tests fail to detect early viral replication preventing early treatment response. Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) over serological testing and polymerase chain reaction (PCR), for the rapid detection of microbial pathogens. Here we developed a rapid, sensitive, and portable system-integrative LAMP assay for the detection of HBV DNA in plasma samples. The final optimized assay was achieved with an amplification time of less than 45 min at 62°C. The assay showed 100% specificity, 92.20% sensitivity, and a detection limit of 10 copies/µL in 77 HBV-positive plasma samples with known Cq values. Our results showed that the colorimetric LAMP assay is sensitive, efficient, and supremely reliable for rapid detection of HBV, and may be potentially used as a screening test in areas with poor laboratory facilities and limited resource availability.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)
dc.identifier.citationAltın, K. H. ve Agel, E. (2024). Development of molecular-based screening test for hepatitis b virus in human plasma samples. Japanese Journal of Infectious Diseases, 77(2), 97-104. http://dx.doi.org/10.7883/yoken.JJID.2023.313
dc.identifier.doi10.7883/yoken.JJID.2023.313
dc.identifier.endpage104
dc.identifier.issn1344-6304
dc.identifier.issn1884-2836
dc.identifier.issue2
dc.identifier.pmid38030268
dc.identifier.scopus2-s2.0-85188664049
dc.identifier.scopusqualityQ3
dc.identifier.startpage97
dc.identifier.urihttp://dx.doi.org/10.7883/yoken.JJID.2023.313
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13136
dc.identifier.volume77
dc.identifier.wosWOS:001312446800006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAltın, Kevser Hanne
dc.institutionauthorid0000-0001-6068-3892
dc.language.isoen
dc.relation.ispartofJapanese Journal of Infectious Diseases
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/20AG004
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMediated Isothermal Amplification
dc.subjectRapid Detection
dc.subjectGenotype
dc.subjectInfection
dc.subjectHbv
dc.titleDevelopment of molecular-based screening test for hepatitis b virus in human plasma samples
dc.typeArticle

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