Consequences of neurite transection in vitro

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dc.authorid0000-0003-0352-1947
dc.contributor.authorCengiz, Nurettin
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorErdo?an, Ender
dc.contributor.authorHim, Aydın
dc.contributor.authorOğuz, Elif Kaval
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:58:47Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:58:47Z
dc.date.issued2012
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000309967300004
dc.descriptionPubMed ID: 20121423
dc.description.abstractIn order to quantify degenerative and regenerative changes and analyze the contribution of multiple factors to the outcome after neurite transection, we cultured adult mouse dorsal root ganglion neurons, and with a precise laser beam, we transected the nerve fibers they extended. Cell preparations were continuously visualized for 24 h with time-lapse microscopy. More distal cuts caused a more elongated field of degeneration, while thicker neurites degenerated faster than thinner ones. Transected neurites degenerated more if the uncut neurites of the same neuron simultaneously degenerated. If any of these uncut processes regenerated, the transected neurites underwent less degeneration. Regeneration of neurites was limited to distal cuts. Unipolar neurons had shorter regeneration than multipolar ones. Branching slowed the regenerative process, while simultaneous degeneration of uncut neurites increased it. Proximal lesions, small neuronal size, and extensive and rapid neurite degeneration were predictive of death of an injured neuron, which typically displayed necrotic rather than apoptotic form. In conclusion, this in vitro model proved useful in unmasking many new aspects and correlates of mechanically-induced neurite injury.
dc.description.sponsorshipYuzuncu Yil University Directorate of Scientific Research Projects [TF073]en_US
dc.description.sponsorshipThis study was supported by the Yuzuncu Yil University Directorate of Scientific Research Projects (grant no. TF073).en_US
dc.identifier.citationCengiz, N., Öztürk, G., Erdo?an, E., Him, A. ve Oğuz, E.(2012). Consequences of neurite transection in vitro. Journal of Neurotrauma, 29(15), 2465-2474. https://dx.doi.org/10.1089/neu.2009.0947
dc.identifier.doi10.1089/neu.2009.0947
dc.identifier.endpage2474
dc.identifier.issn0897-7151
dc.identifier.issue15
dc.identifier.scopusqualityQ1
dc.identifier.startpage2465
dc.identifier.urihttps://dx.doi.org/10.1089/neu.2009.0947
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3227
dc.identifier.volume29
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert Inc
dc.relation.ispartofJournal of Neurotraumaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAxonal Injury
dc.subjectAxonal Regeneration
dc.subjectDegeneration
dc.subjectNeuronal Cell Death
dc.titleConsequences of neurite transection in vitro
dc.typeArticle

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