Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements

Basit Öğe Kaydı
dc.authorid0000-0002-2168-710X
dc.authorid0000-0002-0150-6762
dc.contributor.authorOlcay, Keziban
dc.contributor.authorTaşlı, Pakize Neslihan
dc.contributor.authorPamukçu Güven, Esra
dc.contributor.authorYalçın Ülker, Gül Merve
dc.contributor.authorEsen Öğüt, Emine
dc.contributor.authorÇiftçio?lu, Elif
dc.contributor.authorKıratlı, Binnur
dc.contributor.authorŞahin, Fikrettin
dc.date.accessioned2020-04-22T08:17:27Z
dc.date.available2020-04-22T08:17:27Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Ağız, Diş ve Çene Cerrahisi Ana Bilim Dalı
dc.description.abstractObjective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). Conclusion: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs.
dc.identifier.citationOlcay, K., Taşlı, P. N., Pamukçu Güven, E., Yalçın Ülker, G. M., Esen Öğüt, E., Çiftçio?lu, E. ... Şahin, F. (2020). Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements. Journal of Applied Oral Science, 28. https://dx.doi.org/10.1590/1678-7757-2019-0215
dc.identifier.doi10.1590/1678-7757-2019-0215
dc.identifier.issn1678-7757
dc.identifier.issn1678-7765
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://hdl.handle.net/20.500.12511/5199
dc.identifier.urihttps://dx.doi.org/10.1590/1678-7757-2019-0215
dc.identifier.volume28
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBauru School of Dentistry, University of Sao Paulo
dc.relation.ispartofJournal of Applied Oral Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightshttps://creativecommons.org/licenses/by/4.0/*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAngiogenesis Inducing Agents
dc.subjectDental Cements
dc.subjectRegenerative
dc.subjectEndodontics
dc.subjectStem Cells
dc.titleEffect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Olcay, Keziban-2020.pdf
Boyut:
2.26 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Diş Hekimliği Fakültesi Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu