Bioenergetic shift and proteomic signature induced by lentiviral-transduction of gfp-based biosensors

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dc.contributor.authorBarakat, Sarah
dc.contributor.authorÇimen, Şeyma
dc.contributor.authorMiri, Seyed Mohammad
dc.contributor.authorVatandaşlar, Emre
dc.contributor.authorYelkenci, Hayriye Ecem
dc.contributor.authorSan Martín, Alejandro
dc.contributor.authorBeker, Mustafa Çağlar
dc.contributor.authorKök, Kıvanç
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorEroğlu, Emrah
dc.date.accessioned2025-11-05T12:24:04Z
dc.date.available2025-11-05T12:24:04Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Beslenme ve Diyetetik Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik ve Tıp Bilişimi Ana Bilim Dalı
dc.description.abstractFluorescent proteins (FPs) stand as pivotal tools extensively employed across diverse biological research endeavors in various model systems. However, long-standing concerns surround their use due to the numerous side effects associated with their expression. Recent investigations have brought to light the significance of hydrogen peroxide (H2O2) that is associated with the maturation process of green fluorescent protein (GFP) fluorophores. The structural and functional impairments associated with GFP expression are possibly linked to this amount of H2O2. In this study, we assess the impact of the GFP-based HyPer7 biosensor on cellular homeostasis and proteome changes, aiming to identify potential risks related to oxidative stress responses that potentially risks the application of such tools. Cells expressing genome-integrated HyPer7 demonstrated altered mitochondrial membrane potential (MMP), which was alleviated by the addition of antioxidants or culturing cells at physiological normoxia (5 kPa O2). Additionally, HyPer7-expressing cells also exhibited significant impairment in mitochondrial oxidative respiration, suggesting broader mitochondrial dysfunction. Through untargeted proteomics analysis, we identified 26 proteins exhibiting differential expression in HyPer7-expressing cells compared to respective control cells. Functional annotation analysis showed that the list of the delineated proteins is associated with cellular responses to stress and the regulation of antioxidant mechanisms. Our findings underscore the significance of caution and validation in ensuring a thorough comprehension of cellular responses when using fluorescent protein-based tools, thereby enhancing the reliability of the results.
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO)
dc.identifier.citationBarakat, S., Çimen, Ş., Miri, S. M., Vatandaşlar, E., Yelkenci, H. E., San Martín, A. ... Eroğlu, E. (2024). Bioenergetic shift and proteomic signature induced by lentiviral-transduction of gfp-based biosensors. Redox Biology, 78. http://dx.doi.org/10.1016/j.redox.2024.103416
dc.identifier.doi10.1016/j.redox.2024.103416
dc.identifier.issn2213-2317
dc.identifier.pmid39509993
dc.identifier.scopus2-s2.0-85208110651
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1016/j.redox.2024.103416
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13161
dc.identifier.volume78
dc.identifier.wosWOS:001353500000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBarakat, Sarah
dc.institutionauthorMiri, Seyed Mohammad
dc.institutionauthorVatandaşlar, Emre
dc.institutionauthorYelkenci, Hayriye Ecem
dc.institutionauthorBeker, Mustafa Çağlar
dc.institutionauthorKök, Kıvanç
dc.institutionauthorÖztürk, Gürkan
dc.institutionauthorEroğlu, Emrah
dc.institutionauthorid0000-0001-9788-4153
dc.institutionauthorid0000-0002-4681-9703
dc.institutionauthorid0000-0002-9476-8488
dc.institutionauthorid0000-0002-1502-1600
dc.institutionauthorid0000-0003-0352-1947
dc.language.isoen
dc.relation.ecinfo:eu-repo/grantAgreement/EC/FP7/IG4113
dc.relation.ispartofRedox Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGreen Fluorescent Proteins
dc.subjectHydrogen Peroxide
dc.subjectHyper7 Genetically Encoded Biosensor
dc.subjectOxidative Stress
dc.subjectProteomic Signature
dc.titleBioenergetic shift and proteomic signature induced by lentiviral-transduction of gfp-based biosensors
dc.typeArticle

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