Morphine ameliorates pentylenetetrazole-induced locomotor pattern in zebrafish embryos; mechanism involving regulation of opioid receptors, suppression of oxidative stress, and inflammation in epileptogenesis

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dc.authorid0000-0003-0804-1475
dc.authorid0000-0002-6274-801X
dc.contributor.authorÜstündağ, Fümet Duygu
dc.contributor.authorÜnal, İsmail
dc.contributor.authorÜstündağ, Ünsal Veli
dc.contributor.authorCansız, Derya
dc.contributor.authorBeler, Merih
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorTiber, Pınar Mega
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2023-09-19T10:27:24Z
dc.date.available2023-09-19T10:27:24Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractZebrafish (Danio rerio) is becoming an increasingly important model in epilepsy research. Pentylenetetrazole (PTZ) is a convulsant agent that induces epileptic seizure-like state in zebrafish and zebrafish embryos and is most commonly used in antiepileptic drug discovery research to evaluate seizure mechanisms. Classical antiepileptic drugs, such as valproic acid (VPA) reduce PTZ-induced epileptiform activities. Opioid system has been suggested to play a role in epileptogenesis. The aim of our study is to determine the effects of morphine in PTZ-induced epilepsy model in zebrafish embryos by evaluating locomotor activity and parameters related to oxidant-antioxidant status, inflammation, and cholinergic system as well as markers of neuronal activity c-fos, bdnf, and opioid receptors. Zebrafish embryos at 72 hpf were exposed to PTZ (20 mM), VPA (1 mM), and Morphine (MOR) (100 mu M). MOR and VPA pretreated groups were treated with either MOR (MOR + PTZ) or VPA (VPA + PTZ) for 20 min before PTZ expoure. Locomotor activity was quantified as total distance moved (mm), average speed (mm/sec) and exploration rate (%) and analyzed using ToxTrac tracking programme. Oxidant-antioxidant system parameters, acetylcholinesterase activity, and sialic acid leves were evaluated using spectrophotometric methods. The expression of c-fos, bdnf, oprm1, and oprd1 were evaluated by RT-PCR. MOR pretreatment ameliorated PTZ-induced locomotor pattern as evidenced by improved average speed, exploration rate and distance traveled. We report the restoration of inflammatory and oxidant-antioxidant system parameters, c-fos, bdnf, and opioid receptor oprm1 as the possible mechanisms involved in the ameliorative effect of MOR against PTZ-induced epileptogenic process in zebrafish embryos.
dc.identifier.citationÜstündağ, F. D., Ünal, İ., Üstündağ, Ü. V., Cansız, D., Beler, M., Alturfan, A. A. ... Emekli Alturfan, E. (2023). Morphine ameliorates pentylenetetrazole-induced locomotor pattern in zebrafish embryos; mechanism involving regulation of opioid receptors, suppression of oxidative stress, and inflammation in epileptogenesis. Toxicology Mechanisms and Methods, 33(2), 151-160. https://doi.org/10.1080/15376516.2022.2105182
dc.identifier.doi10.1080/15376516.2022.2105182
dc.identifier.endpage160
dc.identifier.issn1537-6516
dc.identifier.issn1537-6524
dc.identifier.issue2
dc.identifier.pmid35866229
dc.identifier.scopus2-s2.0-85135273210
dc.identifier.scopusqualityQ2
dc.identifier.startpage151
dc.identifier.urihttps://doi.org/10.1080/15376516.2022.2105182
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11457
dc.identifier.volume33
dc.identifier.wos000834891700001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÜstündağ, Ünsal Veli
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofToxicology Mechanisms and Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectMorphine
dc.subjectOxidant-Antioxidant
dc.subjectPentylenetetrazole
dc.subjectValproic Acid
dc.subjectZebrafish Embryos
dc.titleMorphine ameliorates pentylenetetrazole-induced locomotor pattern in zebrafish embryos; mechanism involving regulation of opioid receptors, suppression of oxidative stress, and inflammation in epileptogenesis
dc.typeArticle

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