Assessing the impact of the novel sperm selection technique 'annexin-v coated polystyrene bead technique' on mouse assisted reproductive techniques outcomes: preliminary findings

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dc.contributor.authorKarabulut, Seda
dc.contributor.authorYalım Camcı, İrem
dc.contributor.authorErdem Altun, Ceren
dc.contributor.authorUsta, Melek
dc.contributor.authorYiğit, Pakize
dc.date.accessioned2025-06-12T12:55:08Z
dc.date.available2025-06-12T12:55:08Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik ve Tıp Bilişimi Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractICSI is one of the most commonly used techniques to treat infertility. The sperm selection for the procedure is done ‘randomly’ by the embryologist according to the motility and morphology parameters which is known not to reflect the potential of a sperm for fertilization, pregnancy and a healthy childbearing. Since the apoptosis rate is higher in sperm cells of infertile patients, it is more likely to choose an apoptotic sperm by the 'random selection method'. We recently introduced a novel sperm selection technique namely ‘Annexin-V coated polystrene bead technique’(APB-Tech), for the selection of non-apoptotic sperm cells. The principal of the technique is based on the binding affinity of an apoptotic sperm to ‘Annexin-V covered beads’ enabling to distinguish a viable and a healthy sperm by light microscopy. The aim of the present study was to observe the effects of this technique on ICSI outcomes in mice. Sibling-oocyte trial was conducted and the outcome measures were compared with the results of traditional sperm selection method. Embryo and blastocyst qualities and blastocyst development rates were significantly increased in APB-Tech group, while the other parameters were not affected. Promising results obtained from the technique reflect its promising potential as a new and powerful tool for sperm selection and thus infertility techniques.
dc.description.sponsorshipTÜBİTAK
dc.identifier.citationKarabulut, S., Yalım Camcı, İ., Erdem Altun, C., Usta, M. ve Yiğit, P. (2024). Assessing the impact of the novel sperm selection technique 'annexin-v coated polystyrene bead technique' on mouse assisted reproductive techniques outcomes: preliminary findings. Reproductive Sciences, 31(9), 2794-2807. http://dx.doi.org/10.1007/s43032-024-01620-w
dc.identifier.doi10.1007/s43032-024-01620-w
dc.identifier.endpage2807
dc.identifier.issn1933-7191
dc.identifier.issn1933-7205
dc.identifier.issue9
dc.identifier.pmid38874899
dc.identifier.scopus2-s2.0-85196112514
dc.identifier.scopusqualityQ1
dc.identifier.startpage2794
dc.identifier.urihttp://dx.doi.org/10.1007/s43032-024-01620-w
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12958
dc.identifier.volume31
dc.identifier.wosWOS:001247211700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKarabulut, Seda
dc.institutionauthorErdem Altun, Ceren
dc.institutionauthorUsta, Melek
dc.institutionauthorYiğit, Pakize
dc.institutionauthorid0000-0003-3302-5004
dc.institutionauthorid0000-0002-5919-1986
dc.language.isoen
dc.relation.ispartofReproductive Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAPB-Tech
dc.subjectEmbryo
dc.subjectICSI
dc.subjectInfertility
dc.subjectSperm Selection
dc.titleAssessing the impact of the novel sperm selection technique 'annexin-v coated polystyrene bead technique' on mouse assisted reproductive techniques outcomes: preliminary findings
dc.typeArticle

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