In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model

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dc.contributor.authorKelmanson, Ilya V.
dc.contributor.authorShokhina, Arina G.
dc.contributor.authorKotova, Daria A.
dc.contributor.authorPochechuev, Matvei S.
dc.contributor.authorIvanovaa, Alexandra D.
dc.contributor.authorKostyuka, Alexander I.
dc.contributor.authorPanova, Anastasiya S.
dc.contributor.authorBorodinova, Anastasia A.
dc.contributor.authorSolotenkovc, Maxim A.
dc.contributor.authorStepanov, Evgeny A.
dc.contributor.authorRaevskiia, Roman I.
dc.contributor.authorMoshchenko, Aleksandr A.
dc.contributor.authorPak, Valeriy V.
dc.contributor.authorErmakova, Yulia G.
dc.contributor.authorvan Belle, Gijsbert J.C.
dc.contributor.authorTarabykin, Viktor
dc.contributor.authorBalaban, Pavel M.
dc.contributor.authorFedotov, Ilya V.
dc.contributor.authorFedotov, Andrei B.
dc.contributor.authorConrad, Marcus
dc.contributor.authorBogeski, Ivan
dc.contributor.authorKatschinski, Dörthe M.
dc.contributor.authorDoeppner, Thorsten R.
dc.contributor.authorBähr, Mathias
dc.contributor.authorZheltikov, Aleksei M.
dc.contributor.authorBelousov, Vsevolod V.
dc.contributor.authorBilan, Dmitry S.
dc.date.accessioned2021-11-30T09:13:18Z
dc.date.available2021-11-30T09:13:18Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.description.abstractIschemic cerebral stroke is one of the leading causes of death and disability in humans. However, molecular processes underlying the development of this pathology remain poorly understood. There are major gaps in our understanding of metabolic changes that occur in the brain tissue during the early stages of ischemia and reperfusion. In particular, it is generally accepted that both ischemia (I) and reperfusion (R) generate reactive oxygen species (ROS) that cause oxidative stress which is one of the main drivers of the pathology, although ROS generation during I/R was never demonstrated in vivo due to the lack of suitable methods. In the present study, we record for the first time the dynamics of intracellular pH and H2O2 during I/R in cultured neurons and during experimental stroke in rats using the latest generation of genetically encoded biosensors SypHer3s and HyPer7. We detect a buildup of powerful acidosis in the brain tissue that overlaps with the ischemic core from the first seconds of pathogenesis. At the same time, no significant H2O2 generation was found in the acute phase of ischemia/reperfusion. HyPer7 oxidation in the brain was detected only 24 h later. Comparison of in vivo experiments with studies on cultured neurons under I/R demonstrates that the dynamics of metabolic processes in these models significantly differ, suggesting that a cell culture is a poor predictor of metabolic events in vivo.
dc.identifier.citationKelmanson, I. V., Shokhina, A. G., Kotova, D. A., Pochechuev, M. S., Ivanovaa, A. D., Kostyuka, A. I. ... Bilan, D. S. (2021). In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model. Redox Biology, 48. https://dx.doi.org/10.1016/j.redox.2021.102178
dc.identifier.doi10.1016/j.redox.2021.102178
dc.identifier.issn2213-2317
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1016/j.redox.2021.102178
dc.identifier.urihttps://hdl.handle.net/20.500.12511/8592
dc.identifier.volume48
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofRedox Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGenetically Encoded Fluorescent Biosensors
dc.subjectHydrogen Peroxide
dc.subjectHypoxia/Reoxygenation
dc.subjectIn Vivo Optical Brain Interrogation
dc.subjectIschemia/Reperfusion
dc.subjectIschemic Stroke
dc.titleIn vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model
dc.typeArticle

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